基于转录组测序对茶树GST基因表达的研究

Analysis of Glutathione S-transferase Genes in Tea Plant(Camellia sinensis) Based on Transcriptome Analysis

谷胱甘肽转移酶(Glutathione S-transferases,GSTs)在植物体内普遍存在,是一类具有多种生物学功能的超家族蛋白酶。本研究通过对中黄2号、龙井43在正常光照和遮荫处理下的转录组测序,筛选获得了49个Cs GSTs基因家族成员,并对其中19个在芽叶中表达量较高的Cs GSTs基因进行了序列分析和聚类分析。另外对表达量较高的8个候选基因进行荧光定量表达分析,研究它们在龙井43不同叶位中的表达模式。结果表明这些Cs GSTs基因在一芽一叶到第六叶中均有表达,但各自呈现出不同的变化规律。Cs GST20在龙井43一芽一叶到第六叶中的表达量逐渐上升,可能与植物抗胁迫有关,而Cs GST24的表达量则显著下降,可能与花青素代谢有关。

Glutathione 5-transferases （GSTs） belong to a superfamily of multifunctional enzymes and are ubiquitous in plants. Forty nine CsGSTs genes were identified by transcriptome analysis of Zhonghuang 2 and Longjing 43 under control and shading treatment. Nineteen CsGSTs with relatively high expression levels in buds were used for sequence and phylogenetic tree analysis. Real-time fluorescence quantitative PCR （qRT-PCR） was performed to analyze the expression patterns of 8 candidate genes in different leaf positions of Longjing 43. The results showed that CsGSTs were expressed in all tested leaves,but exhibited different expression patterns. CsGST20 showed an increasing expression from a bud with a leaf to the sixth leaf,suggesting a potential role in stress resistance. On the other hand,the expression level of CsGST24 dropped significantly,suggesting that CsGST24 might participate in anthocyanin accumulation in tea plants.