养心氏片对气虚血瘀证慢性心肌缺血模型大鼠缺血损伤的保护作用

Protective Effects of Yangxinshi Tablet on Chronic Myocardial Ischemia with Qi Deficiency and Blood Stasis Modeel in Rats

目的研究养心氏片对气虚血瘀证慢性心肌缺血模型大鼠的保护作用。方法将60只健康Wistar雄性大鼠随机分为6组,分别为假手术组、模型组、阳性对照组及养心氏片高、中、低剂量组,连续给药28d后,采用多因素复制慢性心肌缺血气虚血瘀大鼠模型,检测心肌梗死面积,测定各组血清中肌酸激酶(CK)、乳酸脱氢酶(LDH),心肌超氧化物歧化酶(SOD)、丙二醛(MDA)的含量变化。结果养心氏片能明显改善模型大鼠心肌缺血程度,减少心肌梗死面积,降低模型大鼠的CK,LDH,MDA含量,升高SOD含量。结论养心氏片能增加抗氧化酶活性,提高缺血心肌细胞的抗氧化能力,减少氧自由基对心肌的氧化损伤,对慢性心肌缺血气虚血瘀模型动物缺血损伤有保护作用。

Objective To study the protective effects of Yangxinshi tablet（YXST）on chronic myocardial ischemia with qi deficiency and blood stasis syndrome in rats.Methods Sixty wistar male rats were randomly divided into six groups：sham group,model group,positive control group,YXST high dose group,medial dose group and low dose group.After twenty-eight days of continuous administration,rat with chronic myocardial ischemia with qi deficiency and blood stasis syndrome was made by multiple factors way,then myocardial infarct size was measured.The activities of creatine kinase（CK）,lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,malonaldehyde（MDA）were determined in each groups.Results The models＇ Σ-ST improved,myocardial infarction size reduced,obviously activities of CK,LDH,MDA decreased,and the contents of SOD increased in YXST groups.Conclusion YXST shows protective effects on Chronic myocardial ischemia with Qi deficiency and blood stasis syndrome.