丹皮酚对大鼠冠状动脉粥样硬化的影响

Influence of paeonol on coronary atherosclerosis

目的讨论丹皮酚对大鼠冠状动脉粥样硬化的影响。方法选择健康雄性SD大鼠46只,所有大鼠随机分为6组,正常组(7只)、模型组(8只),低剂量、中剂量、高剂量组各8只,辛伐他汀组(阳性对照)7只。正常组普通饲料喂养,生理盐水灌胃,连续8周;模型组:高脂饮食25 g/只/d饲喂,连续8周,以等体积的生理盐水灌胃,连续8周;低剂量、中剂量、高剂量组:大鼠高脂饮食25 g/只/d,连续8周,然后给予不同剂量(低、中、高)的丹皮酚(30、60、120 mg/kg·d)灌胃,连续8周;辛伐他汀组:高脂饮食25 g/只/d,连续8周,然后辛伐他汀10 mg/kg·d灌胃,连续8周。RT-PCR和琼脂糖凝胶电泳检测甘油醛-3-磷酸脱氢酶(GAPDH)、细胞间黏附分子-1(ICAM-1)、血管细胞粘附分子-1(VCAM-1)、单核细胞趋化因子-1(MCP-1)表达量。全自动生化分析仪检测总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平。结果与正常组比较,模型组大鼠TC、TG、LDL-C以及LDL-C/HDL-C比值升高,HDL-C降低,差异有统计学意义(P均<0.05)。与模型组比较,辛伐他汀组各血脂指标均改善,同时,高剂量组的各血脂指标也改善,差异有统计学意义(P均<0.05)。中剂量组较模型组TC和LDL-C/HDL-C比值降低,差异有统计学意义(P均<0.05)。模型组较对照组ICAM-1、VCAM-1、MCP-1表达均增加,差异有统计学意义(P均<0.05)。与模型组比较,辛伐他汀组ICAM-1、VCAM-1、MCP-1表达均降低,丹皮酚干预的高剂量组ICAM-1、VCAM-1、MCP-1表达也降低,差异有统计学意义(P均<0.05)。结论高剂量丹皮酚明显改善冠状动脉粥样硬化大鼠血脂,降低冠状动脉粥样硬化相关趋化因子的表达,同时降低冠状动脉粥样硬化的风险。

Objective To discuss the influence of paeonol on coronary atherosclerosis in rats. MethodsMale healthy SD rats（n=46） were chosen and randomly divided into 6 groups： normal group（normal forage and normal saline solution for 8 w, n=7）, model group（25 g/rat/d of high-fat forage for 8 w and normal saline solution for 8 w, n=8）, low-dose group（25 g/rat/d of high-fat forage for 8 w and 30 mg/kg·d of paeonol for 8 w, n=8）, middose group（25 g/rat/d of high-fat forage for 8 w and 60 mg/kg·d of paeonol for 8 w, n=8）, high-dose group（25 g/rat/d of high-fat forage for 8 w and 120 mg/kg·d of paeonol for 8 w, n=8） and control group（25 g/rat/d of highfat forage for 8 w and 10 mg/kg·d of simvatatin for 8 w, n=7）. The expressions of GAPDH, intercellular adhesion molecule-1（ICAM-1）, vascular adhesion molecule-1（VCAM-1） and monocyte chemoattractant protein-1（MCP-1） were detected by using real-time fluorescence quantitative polymerase chain reaction（RT-PCR） and agarose gel electrophoresis. The levels of total cholesterol（TC）, triglyceride（TG）, low-density lipoprotein-cholesterol（LDL-C） and high-density lipoprotein-cholesterol（HDL-C） were detected by using automatic biochemical analyzer. ResultsCompared with normal group, the levels of TC, TG and LDL-C and ratio of LDL-C to HDL-C increased, and level of HDL-C decreased in model group（all P〈0.05）. Compared with model group, all blood fat indexes were improved in control group and in high-dose group at the same time（all P〈0.05）. The level of TC and ratio of LDL-C to HDL-C decreased in mid-dose group compared with model group（all P〈0.05）. The expressions of ICAM-1, VCAM-1 and MCP-1 increased in model group compared with control group（all P〈0.05）. Compared with model group, the expressions of ICAM-1, VCAM-1 and MCP-1 decreased in control group and high-dose group（all P〈0.05）. Conclusion Paeonol in high dose can significantly improve blood fat indexes, reduce the expressions of chemotactic factors related to coronary atherosclerosis and decrease risk of coronary atherosclerosis.