Th17细胞在不同国际预后指数组DLBCL中的表达及意义

Expression of Th17 cells in different DLBCL IPI groups and its significance

目的探讨初诊的弥漫大B细胞淋巴瘤(DLBCL)患者外周血Th17细胞的表达与国际预后指标(IPI)之间关系。方法初诊DLBCL组(n=45)按照国际预后指数(IPI)积分分为4组,采用ELISA和流式细胞术检测各个DLBCL组与正常对照组(n=43)的外周血中IL-17的浓度以及Th17阳性细胞比例,比较各组数值间的差异,并分析IPI的5个指标与IL-17的浓度以及Th17阳性细胞比例的相关性。结果 DLBCL中高危组与高危组的IL-17的浓度以及Th17阳性细胞比例较正常对照组及其他IPI组降低,有显著性差异;DLBCL四组的IL-17的浓度以及Th17阳性细胞比例均低于正常对照组;且可见随着IPI分组的增高,IL-17的浓度以及Th17阳性细胞比例呈降低的趋势;IPI指标中年龄、临床分期、全身状态与Th17细胞的表达有相关性。结论初诊DLBCL患者外周血Th17细胞的表达与国际预后指标有关系;随着IPI积分的增加,DLBCL患者Th17细胞表达下降;临床上对于年龄60岁以上、临床分期Ⅲ期以上、长期卧床及需别人照顾的患者更要注意监测其外周血Th17细胞的表达情况。

Objective To explore the relationship between the international prognosis indexes（ IPI） and the Th17 cells expression in DLBCL patients. Methods DLBCL patients（ n = 45） were divided into 4 groups according to IPI score,peripheral blood were taken from each person in DLBCL groups and normal group. We used ELISA to test IL-17 and flow cytometry（ FCM） to examine the Th17 positive cells. We compared the value of each group,and analyzed the relativity of IPI and Th17cells＇ expression. Results Th17 cells＇ expression level in middle-high risk group and high risk group were higher than that in normal group and other IPI groups; Th17 cells＇ expression level in DLBCL groups were all lower than that in normal group; Th17 cells decreased while IPI score increased; Age,clinical stage and general body state have the relativity with DLBCL patients＇ Th17 cells expression level. Conclusion Th17 cells in DLBCL patients has the relativity with prognosis index. In our clinical diagnosis and treatment,we need to pay more attention to those patients who are over 60 years old,or whose clinical stage is above Ⅲ phase,or who need to stay on bed for a long time and need other peoples＇ help.