白藜芦醇对大鼠肾脏缺血再灌注损伤的保护作用及机制研究

Protective Effects and Mechanism of Resveratrol on Renal Ischemia-Reperfusion Injury in Rats

目的:研究白藜芦醇在肾脏缺血-再灌注损伤中的保护作用及其相关机制。方法:24只雄性SD大鼠随机分为假手术组(S组)、缺血-再灌注组(I/R组)、白藜芦醇后处理组(Res组),每组8只。建立急性肾脏缺血-再灌注损伤模型,于再灌注6h后收集动物血液和肾脏标本。检测血清肌酐(Cr)、尿素氮(BUN)和炎症因子IL-6、IL-12、IL-10、TGF-β浓度,并观察肾脏病理学变化。结果:白藜芦醇后处理可以降低肾脏功能学指标Cr和BUN的浓度,减轻肾脏病理损伤;与I/R组比较,Res组大鼠血清中抗炎因子IL-10和TGF-β含量升高,而促炎因子IL-6和IL-12含量降低,结果具有统计学意义(P<0.05)。结论:白藜芦醇能通过抑制促炎因子和促进抗炎因子释放发挥抗大鼠肾脏缺血-再灌注损伤的作用。

Objective：To evaluate the effect of resveratrol on renal ischemia-reperfusion injury in rats.Methods：24male Sprague-Dawley rats were randomly divided into 3groups（n=8rats in each group）：sham operation group（S）,ischemia/reperfusion injury group（I/R）,post-disposal treatment of resveratrol group（Res）.Renal ischemia/reperfusion model was established.The blood sample and renal tissue samples were taken 6hlater of reperfusion for determination of serum Cr and BUN concentrations.The serum content of IL-6,IL-12,IL-10 and TGF-βwere detected by ELISA.The pathological changes of the kidney were assessed.Results：Compared with I/R,resveratrol post-disposal can alleviate the pathological damage of kidney tissue.It could also reduce the concentration of serum Cr and BUN.Compared with the I/R group,IL-10 and TGF-βlevels dramatic increased while IL-6and IL-12 levels obviously declined in the Res groups.In addition,the results were statistically different（P〈0.05）.Conclusion：Resveratrol can protect kidney against I/R injury by restraining the release of proinflammatory cytokine and promoting the release of anti-inflammatory cytokine.