Gadd45α在不同幽门螺杆菌感染状态胃癌中的差异表达及临床意义

Differential expression of Gadd45α in Helicobacter Pylori-infected human gastric cancer and clinical significance

目的:通过分析Gadd45α在幽门螺杆菌(HP)阳性和阴性胃癌组织中的差异表达来探讨其对胃癌发生的意义,为胃癌的临床诊断和治疗提供理论依据。方法:采用免疫组化方法对选取的70例不同HP感染状态的胃癌及56例癌旁正常组织的石蜡标本中Gadd45α蛋白的表达情况进行检测,分析Gadd45α蛋白的阳性表达与胃癌各项临床病理指标的关系;并分别采用Western blot、反转录PCR(RT-PCR)方法检测选取的8对胃癌活体标本中Gadd45α蛋白及m RNA的表达情况。结果:免疫组化检测结果显示,Gadd45α+-+蛋白在HP胃癌、HP胃癌和HP癌旁组织中的表达率分别是77.5%、46.67%和34.21%,其差异有统计学意义(P<0.05);Gadd45α蛋白在胃癌组织中表达阳性率为64.29%(45/70),与肿瘤大小相关。胃癌组织中Gadd45α蛋白的表达与HP感染状态呈正相关(r=s+0.318),经Spearman秩相关分析提示有统计学意义(P<0.05)。Western blot和RT-PCR法检测结果显示,在HP胃癌组织活体标本中+Gadd45α的蛋白和m RNA表达水平大多高于相应的非癌组织。结论:Gadd45α蛋白在胃癌(尤其HP胃癌)中高表达,其表达水平与胃+癌(尤其HP胃癌)的发生、发展关系密切,对其表达水平的检测有益于胃癌的早期诊断及其预后的早期预测。在胃癌组织中,+Gadd45α蛋白与HP的表达具有正相关性,提示两者在胃癌的发生、发展过程中有一定的相互作用,通过对HP胃癌患者的抗HP治疗可能影响患者的预后。

OBJECTIVE： To investigate the differential expression level of Gadd45α in human gastric cancer with Helicobacter Pylori （HP） infection status and the clinical significance of the expression for improved clinical diagnosis and treatment for gastric cancer. METHODS ：70 paraffin-embeded samples of gastric cancerwith HP infection status and 56 normal tissues from surgically resected tissues were selected at random. The expressions of Gadd45α protein in the samples were detected by immuno-histochemical staining. The relationship between the positive expression of Gadd45α protein and the clinico-pathological parameters of gastric cancer was analyzed. Furthermore,expression levels of Gadd45α mRNA and protein in 8 fresh gastric cancer specimens were detected using Western blot and RT-PCR. RESULTS： Immuno-histochemical analyses showed that the positive expression rate of Gadd45α protein in HP＋ gastric carcinoma tissues,HP gastric carcinoma tissues and HP＋ adjacent non-neoplastic tissues were 77.5%,46.67% and 34.21%,respectively. Their differences were significant （P〈0.05）. The expression rate in gastric carcinoma tissues was 64.29% and it was correlated with tumor size. The expression of Gadd45α protein in gastric carcinoma was positively correlated with HP infection status （rs=0.318, P〈0.05） by a correlated statistical analysis of Spearman’s Rho. Western blot and RT-PCR detection results showed that HP＋ gastric cancer tissues had a higher expression in protein and mRNA levels of Gadd45α in fresh specimens than in adjacent non-neoplastic tissues. CONCLUSION ：Expression of Gadd45α proteinwas up-regulated,especially in HP＋ gastric carcinomas. The expression level was closely related to the occurrence and development of gastric carcinoma （especially HP＋）. The detection of the expression of Gadd45α protein was helpful for early diagnosis and for prediction of prognosis of gastric carcinoma. The expression of Gadd45α protein and Helicobacter Pylori infection status in gastric carcinoma were positively correlated. This was probably correlated with the development of gastric carcinoma. The prognosis of HP＋ gastric carcinoma might be affected by anti HP treatment.