维吾尔族妇女宫颈癌患者的免疫状态及其与临床病理因素的关系

Association between immune state and clinical pathologic parameters in cervical cancer of Uighur women

目的:利用新疆特有的病例资源,评估维吾尔族妇女宫颈癌患者的免疫水平,探讨宫颈癌患者血浆中白细胞介素(IL-2和IL-10)的表达水平及其与临床病理因素之间的关系。方法:收集宫颈癌50例、癌前病变(CINⅢ)患者20例及对照组(正常或慢性宫颈炎患者)18例的外周血标本,使用酶联免疫吸附实验(ELISA)法检测血浆中细胞因子IL-2和IL-10的表达水平。结果:与对照组相比较,宫颈癌和癌前病变组IL-2的表达水平显著降低(P<0.05),IL-10的表达水平显著升高(P<0.05);在宫颈癌组中,IL-10的表达水平随肿瘤分期逐渐升高(P<0.05);对照组中汉族与维吾尔族人群血浆中IL-2表达水平无显著差异(P>0.05),宫颈癌组中维吾尔族患者血浆IL-2表达水平较汉族患者显著降低(P<0.05)。结论:宫颈癌患者细胞免疫水平低下,体内发生免疫抑制,提示这可能是宫颈癌细胞发生免疫逃逸的机制之一,IL-2可能在维吾尔族宫颈癌发生发展过程中起到重要作用。

OBJECTIVE： The aim of this study was to investigate the association between IL -2 and IL -1 0cytokine levels in plasma,and clinical pathologic factors in cervical cancer of Uighur women. METHODS： Peripheral blood specimens from 50 cervical cancer patients,20 patients with CIN III precancerous lesions and 18 controls were used to detect IL-2 and IL-10 by ELISA. R E SU LTS ：The results show that expressions of IL -2 in plasma from patients with cervical cancer or precancerous lesions was significantly reduced compared with those from the controls （P〈0.05）,while expressions of IL-10 was significantly increased （P〈0.05）. From the cervical cancer group,IL -10 expression was gradually increased with tumor pathological staging （P〈0.05）,while IL-2 expression level did not show obvious relationship with tumor pathological staging （P〉0.05）. Moreover,there was no significant difference in IL-2 expression （P〉0.05） between normal Han and Uighur populations,but among those with cervical cancer,Uighur patients’ IL-2 expression level was significantly lower than that in Han patients （P〈0.05）. CONCLUSION ：Our results show that immunosuppression occurred despite having low cellular immune level among cervical cancer patients. The observation suggests that one of the mechanisms may be immune escape of tumor cells. In addition,IL -2 may have played an important role in cervical carcinogenesis among Uighur women.