季节对狐狸精子发生的影响

Seasonal changes in spermatogenesis in silver fox

目的:狐狸是短日照季节性单次发情动物,精子的发生有明显的阶段特异性。本研究是为了探明季节变化对狐狸精子发生的影响。方法:以未发情期(2014年9月28日)、睾丸发育初期(2014年11月29日)和睾丸发育成熟期(2015年1月27日)的银狐各2只为研究对象,称量双侧睾丸的质量和体积,采用酶联免疫吸附实验检测睾丸中睾酮的含量。并利用组织切片技术分析曲细精管中各类生精细胞的组成及比例。结果:9月、11月和次年1月银狐睾丸的质量分别为(0.896±0.002)g、(3.365±0.081)g、(5.241±0.100)3 3 3g;体积分别为(1.215±0.036)cm、(5.558±0.794)cm、(14.112±2.854)cm;睾酮含量分别为(0.211±0.060)μg/g、(2.040±0.202)μg/g、(3.240±0.432)μg/g。从9月至次年1月银狐睾丸质量、体积以及睾酮含量均逐渐增加,组间差异具有统计学意义(P均<0.05)。切片上单个曲细精管横截面中生精细胞的组成和数量随着时间的推移逐渐增加呈现季节变化,组间差异具有统计学意义(P<0.05)。结论:狐狸的精子发生呈现典型的季节性,在皖北采集公狐精液的最佳时间为1月20日左右。本研究为探究银狐的繁殖特性及提高银狐的人工授精率奠定了基础。

OBJECTIVE ： Fox is a seasonal single-estrus animal and its spermatogenesis is stage-specific. The objective of our study was to ascertain the impact of seasonal variations on silver fox spermatogenesis. METHODS ：Silver fox testicles were taken in September,November and January for measurement of testicular volume,weight and other basic morphological parameters. Testosterone in the testes was measured by enzyme-linked assay. Tissue slice seminiferous tubules from all kinds of raw composition and proportion of sperm cells were analyzed. R E SU LTS ：In September,November,January ,the average testicular weights were （0 .896 ±0 .002 ） g , （3.365 ± 0 .081 ） g and （5.241 ±0.100） g; volume were （1.215±0.036） cm^3, （5 .558± 0 .794 ） cm3 and （14 .112± 2 .854 ） cm^3 and testosterone contentswere （0.211±0.060） μg/g,（2 .0 4 0 ± 0 .2 0 2 ） μg/g,（3 .240 ± 0.432 ）μg /g,respectively. Testicular volume,weight and testosterone concentrations in the three months were significantly different （P〈0.05）. The content of testosterone and the weight changes were positively correlated （r=0.991）. In the different seasons,the composition and number of the spermatogenic cell in seminiferous tubules changed with the seasons significantly （P〈0.05）. CONCLUSION ：Our results indicate that spermatogenesis in silver fox showed typical seasonal patterns. The information can be used to determine the best time of semen collection and the approach to enhance artificial fertilization rate.