探讨急性脑血管病患者血清及脑脊液中S-100β蛋白水平变化及临床意义

Changes and Clinical Significance of S-100 β Protein in Serum and Cerebrospinal Fluid of Patients with Acute Cerebral Vascular Disease

目的探讨急性脑血管病患者不同病情程度血清及脑脊液(CSF)中S-100β蛋白动态检测中的临床意义,为早期认知功能评估及治疗提供理论依据。方法自2013年5月—2015年4月,整群选取该院采用荧光免疫测定方法(FIA)测定157例患者(患者组),其中缺血性中风组89例、出血性中风组68例在不同病情程度血清S-100β蛋白的水平变化,同时测定100名健康查体者(对照组)血清及CSF中S-100β蛋白的含量变化。结果缺血性中风组血清及CSF中S-100β蛋白分别为(2.89±1.51)、(4.31±1.92)μg/L,出血性中风组血清及CSF中S-100β蛋白(μg/L)分别为(3.67±1.75)、(6.01±2.13)μg/L;对照组血清及CSF中S-100β蛋白分别为(0.195±0.081)、(3.05±0.418)μg/L。急性脑血管病患者组与对照组血清及CSF中S-100β蛋白含量差异有统计学意义(P<0.001)。不同病情缺血性中风患者(轻度、中度、重度)和出血性中风组(轻度、中度、重度)组内之间血清中及CSFS-100β蛋白的水平变化差异有统计学意义(P<0.001)。结论测定急性脑血管病患者血清及CSF中S-100β蛋白含量变化,对急性脑血管疾病早期诊断及康复治疗提供理论依据,具有重要的临床研究和应用价值。

Objective To investigate the clinical significance of dynamic detection of S-100β protein in serum and cerebrospinal fluid（CSF） in patients with acute cerebrovascular disease,and to provide theoretical basis for early cognitive function assessment and treatment.Methods Group selection since May 2013 to April 2015,by fluorescence immunoassay（FIA） method for the determination of 157 patients（patient group）,including ischemic stroke group 89 cases,hemorrhage stroke group 68 cases in different severity of serum S-100β protein levels and simultaneous determination of 100 cases of health examination persons（control group） of S-100βprotein in serum and CSF of content change.Results The CSFS-100βacute,were（2.89±1.51）,（4.31±1.93）and bleeding stroke serum（3.67±1.75）,（6.01±2.13）.In the control group,the serum and CSF S-100β protein（μg/L） were（0.195±0.081）、（3.05±0.418）.cerebrovascular disease patients group and the control group,the serum and CSF levels of S-100 β protein the difference was statistically（P〈0.001）.And different condition of ischemic stroke patients（mild,moderate,severe） and hemorrhagic stroke group（mild,moderate,severe） within the group between serum and protein level changes the difference was statistically（P〈0.001）.Conclusion To determine the content of S-100 beta protein in serum and CSF in patients with acute cerebrovascular disease,and to provide theoretical basis for early diagnosis and rehabilitation treatment of acute cerebrovascular disease,and has important clinical research and application value.