福辛普利保护高尿酸血症性肾病大鼠的疗效分析

Analysis of the Therapeutic Effect of Fosinopril on the Protective Effect of Hyperuricemia Nephropathy in Rats

目的分析研究福辛普利对高尿酸血症性肾病大鼠血浆血管紧张素Ⅱ(AngⅡ)和醛固酮(ALD)水平的表达及肾间质纤维化的影响,从而探讨其在高尿酸血症性肾病大鼠中的保护作用。方法 2014年6月—2015年6月间在厦门大学医学院动物实验中心选取清洁型SD大鼠80只,随机分为造模组60只,对照组20只;高尿酸血症性肾病大鼠模型的建立采用腺嘌呤+盐酸乙胺丁醇灌胃法,造模成功后将造模组动物随机分为模型组28只、福辛普利干预组28只。于实验第45﹑60天分别检测3组大鼠血浆Ang II、ALD的表达,免疫组化法测定肾脏组织a-平滑肌肌动蛋白(aSMA)的表达。结果 (1)实验第60天福辛普利干预组治疗后血浆AngⅡ(68.75±20.56)pg/m L、ALD(246.56±59.69)pg/m L与同期模型组(86.56±23.30)pg/m L、(346.64±86.78)pg/m L比较有下降(P<0.05);(2)福辛普利干预组治疗后实验第45 d、60 d肾组织中a-SMA的浓度(0.27±0.16)、(0.33±0.56)与模型组(0.52±0.12)、(0.63±0.42)比较均有明显下降(P<0.05)。结论福辛普利可能通过下调高尿酸血症性肾病大鼠模型血浆AngⅡ、ALD水平的表达,抑制肾脏炎症及肾素-血管紧张素系统活化,从而防治高尿酸血症性肾间质纤维化。

Objective To observe the effect of fosinopril on the expression of plasma angiotensin II（Ang II） and aldosterone（ALD） and renal interstitial fibrosis in rats with hyperuricemia nephropathy,Then investigate the protective effect of it in rats with hyperuricemia nephropathy.Methods From June 2014 to June 2015,in the center of animal experiment, xiamen university school of medicine, 80 clean SD rats were randomly divided into 60 groups and the control group of 20;Adenine ＋ethambutol hydrochloride by gastric method is used to establish the rat model of high uric acid nephropathy, After successful modeling, the model animals were randomly divided into Model group 28 and fosinopril intervention group 28.The expression of Ang II and ALD in rat plasma was detected at forty-fifth and sixtieth days, respectively,and the expression of asmooth muscle actin（a-SMA） in renal tissue was determined by immunohistochemistry.Results（1）sixty days fosinopril intervention group after treatment plasma Ang II（68.75 ± 20.56）pg/m L, ALD（246.56 ± 59.69）pg/m L compared with the same period model group（86.56± 23.30） pg/m L、（346.64±86.78） pg/m L compared with the decline（P〈0.05）;（2）fosinopril interven-tion group after treatment for forty-fifth days, sixty days, the concentration of a-SMA in renal tissue（0.27 ± 0.16）,（0.33±0.56） and model group（0.52 ± 0.12）,（0.63 ± 0.42） were significantly decreased（P〈0.05）. Conclusion Fosinopril may be down regulation of hyperuricemia nephropathy rat model of plasma Ang II, ALD levels of expression,Suppression of renal inflammation and activation of the renin angiotensin system,Therefore, the prevention and treatment of hyperuricemia renal interstitial fibrosis.