上海市动物源性大肠杆菌的分离鉴定和药敏试验

Isolation,Identification and Antimicrobial Susceptibility Test to Escherichia coli of Animal Origin in Shanghai City

为掌握上海市动物源性大肠杆菌的耐药情况及耐药规律,对2013—2015年在门诊畜禽中分离到的大肠杆菌进行药敏试验。共分离到236株大肠杆菌,其中猪源164株、禽源72株。选用15种抗生素,采用CLSI推荐的微量肉汤稀释法,对大肠杆菌进行药物敏感试验。结果表明,无论是猪源大肠杆菌,还是禽源大肠杆菌,对大多数抗生素的耐药程度均较高。其中,对磺胺异噁唑、复方新诺明、恩诺沙星和大观霉素等抗生素的耐药情况较为严重;对丁胺卡那霉素和阿莫西林/棒酸2种抗生素较为敏感,耐药率低于20%。从耐药谱型来看,猪源大肠杆菌较禽源大肠杆菌具有更加复杂的耐药谱型。

In order to investigate the antibiotic resistance of Escherichia coli of animal origin in Shanghai, antimicrobial susceptibility test of 236 E.coli isolates from animal clinics to 14 antimicrobial agents was surveyed. Among them,164 strains were identified as swine origin,72 strains were identified as avian origin. Broth microdilution method according to the recommendation of the CLSI was used. The results showed that both E. coli from swine and avian showed antibiotic resistance to most antimicrobial agents. E.coli strains were highly resistant to sulfisoxazole,sulfamethoxazole,enrofloxacin and spectinomycin,etc. Lower antibiotic resistance rate（ ≤ 20%） against amikacin and amoxicillin or clavulanate was showed. In addition,E.coli from swine had a more complex type of drug resistance spectrum than E.coli from avian.