部分集约化养禽场肉鸡马立克氏病横断面调查

Cross-sectional Study on Marek's Disease of Broilers in Some Large Intensive Poultry Farms

为了解肉鸡马立克氏病的流行特征,从6个集约化养殖场随机选择未免疫马立克氏病疫苗的20日龄和45日龄肉鸡各360只,分别采集其羽髓和血清样品,用琼脂免疫扩散试验和PCR试验进行马立克氏病检测。琼脂免疫扩散试验检测结果为：20日龄肉鸡马立克氏病阳性率为3.33%-6.67%,45日龄肉鸡马立克氏病阳性率为5.00%-8.33%;PCR试验检测结果为：20日龄肉鸡马立克氏病阳性率为5.00%-10.00%,45日龄肉鸡马立克氏病阳性率为8.33%-13.33%。统计这两种试验均为阳性的数据,结果显示20日龄肉鸡双阳性率为3.33%-5.00%,45日龄肉鸡双阳性率为5.00%-6.67%。检测结果表明,所调查的肉鸡群存在一定的马立克氏病感染,提示要加强肉鸡马立克氏病防疫,降低其感染发病率。

To investigate the epidemiological characteristics of Marek＆#39;s disease（MD）in broilers,samples of feather pulps and serum were randomly collected respectively from 360 20-day-old broilers and 360 45-day-old broilers,which were derived from 6 un-vaccinated farms. Then the samples were detected for MD by agar gel immunodiffusion test （AGID）and PCR. The AGID results showed that the positive rate of MD was 3.33%-6.67%in 20-day-old broilers and that of the 45-day-old was 5.00%-8.33%;Corresponding results by PCR were 5.00%-10.00%and 8.33%-13.33%respectively. Statistical data showed that the MD positive test by both tests were 3.33%-5.00%in 20-day-old broilers and 5.00%-6.67% in the 45-day-old. The results indicated that the selected groups were infected with MD in some extent,and more effort should be done to reduce the incidence of MD infection in broilers.