益气养阴消癥通络中药对早期糖尿病大鼠肾小球滤过屏障裂孔膜蛋白的影响

Yiqi Yangyin Xiaozheng Tongluo Decoction on CD2AP and WT-1 in glomerular filtration barrier of rats with diabetic nephropathy at early stage

目的探讨益气养阴消癥通络中药对糖尿病大鼠肾小球滤过屏障的影响。方法选择雄性SD大鼠110只,随机分为对照组、模型组、厄贝沙坦组和中药低、中、高剂量[5.29、10.58、21.16 g/(kg·d)]组。用左肾切除术加腹腔注射链脲佐菌素(STZ)制备糖尿病肾病模型,对照组只行左肾切除术。厄贝沙坦组大鼠给予厄贝沙坦15 mg/(kg·d)灌胃,益气养阴消癥通络中药低、中、高剂量组分别灌胃给药,对照组、模型组大鼠等剂量生理盐水灌胃,连续6周。实验结束时用PCR及Western blot印迹法测定肾组织CD2相关蛋白(CD2AP)及wilms'肿瘤抑制基因(WT-1)表达。结果与模型组比较,中药低、中、高剂量组和厄贝沙坦组CD2AP、WT-1表达显著增加(P<0.01)。结论益气养阴、消癥通络中药可保护肾小球滤过屏障外层裂孔膜和足细胞的结构与功能,延缓糖尿病肾病的发生发展。

Objective To investigate the effect of Yiqi Yangyin Xiaozheng Tongluo（ replenishing qi and nourishing yin and resolving mass and freeing collateral vessels） Decoction（ here in after called ＂TCM＂）,a Chinese herbal medicine compound,on glomerular filtration barrier of rats with diabetic nephropathy（ DN） at early stage. Methods Total 110 male SD rats were randomly divided into control group,model group,irbesartan group（ 15 mg/kg）,TCM low-dose,mid-dose and high-dose group at dose of 5. 29,10. 58 and 21. 16 g/kg respectively. DN rat model was induced by intraperitoneal injection of streptozotozin（ STZ） with left nephrectomy. Rats in control group were only performed left nephrectomy. Each medication group was intragastrically administrated（ i. g.） corresponding drugs,and the control group and model group were adminstrated with the same volume of normal saline,for consecutive six weeks. Then protein expressions of CD2 AP and WT-1 were detected by using PCR,and mRNA expressions were determined by Western blotting. Results Compared with model group,mRNA and protein expressions of CD2 AP and WT-1 in all TCM groups and irbesartan group increased significantly（ P〈0. 01）. Conclusion Yiqi Yangyin Xiaozhou Tongluo Decoction protected the glomerular filtration barrierin the outer membrane and structure and function of podocytes,and slowed down the development of DN.