生脉散中人参、麦冬和五味子提取物体外抗氧化作用

Antioxidative Effect in Vitro of Ginseng Radix et Rhizoma,Ophiopogonis Radix and Schisandrae Chinensis Fructus from Shengmai San

目的:明确生脉散中人参、麦冬和五味子提取物的体外抗氧化作用。方法:采用超氧阴离子(O_2^-·),过氧化氢(H_2O_2)和过氧硝基(ONOO^-)生成体系,以离线方式分析生脉散中的人参、麦冬和五味子清除3种活性氧(ROS)的作用,并在此基础上,采用HPLC-鲁米诺-H_2O_2-发光强度(CL)和HPLC-鲁米诺-邻苯三酚-CL,HPLC-鲁米诺-ONOO^--CL联用技术在线分析生脉散中的人参、麦冬和五味子直接清除O_2^-·,H_2O_2和ONOO^-的作用,明确生脉散直接清除ROS的物质基础和抗氧化作用特点。结果:生脉散中人参、麦冬和五味子对于3种ROS具有直接清除作用的活性成分主要集中在亲水性部位,亲脂性部位成分直接清除ROS作用较弱。结论:通过3种ROS在线清除体系,明确了生脉散中人参、麦冬和五味子中直接清除ROS的成分,且直接清除ROS可能并不是生脉散的主要作用,为后期生脉散在心肌缺血损伤中作用机制的研究提供依据以及新思路。

Objective： To clarify the antioxidative effects in vitro of Ginseng Radix et Rhizoma,Ophiopogonis Radix and Schisandrae Chinensis Fructus from Shengmai San. Method： With the use of superoxide anion（ O2-·） H2O2 and ONOO- generation system,reactive oxygen species（ ROS） scavenging effect of Ginseng Radix et Rhizoma, Ophiopogonis Radix and Schisandrae Chinensis Fructus was analyzed in offline mode;furthermore,three online ROS systems including HPLC-luminol-H2O2-CL,HPLC-luminol-pyrogallol-CL,HPLCONOO--luminol-CL were used to analyze the direct scavenging effect of Ginseng Radix et Rhizoma,Ophiopogonis Radix and Schisandrae Chinensis Fructus on O2-·,H2O2 and ONOO-,and clarify its effective components and antioxidative characteristics. Result： Water-soluble components could be the main constituents in scavenging three ROS,and lipophilic component exerted little effect on scavenging ROS. Conclusion： Three constituents with direct ROS scavenging effect were clarified with use of 3 ROS online scavenging systems,and the effect of directly scavenging ROS could be not the main effect of Shengmai San,providing basis and new thoughts for further study on mechanism of Shengmai San in preventing and treating myocardial ischemia injury.