枯草芽孢杆菌对大肠杆菌K88感染仔猪小肠健康的影响

Effects of Bacillus subtilis on the Small Intestine Healthy of Piglet Administered with Escherichia coli K88

试验主要探讨饲粮中添加枯草芽孢杆菌对大肠杆菌K88感染仔猪小肠发育和微生物区系的影响。选取体重(6.8±0.5)kg的杜长大三元杂种去势公仔猪8头,随机区组均分为2组,每组4个重复,每头猪单栏饲养,对照组饲喂基础饲粮,试验组饲喂基础饲粮+0.1%枯草芽孢杆菌制剂,饲养试验结束前每头猪灌服致病性大肠杆菌K88菌液(1×1011 CFU)。试验结果表明,试验组仔猪比对照组显著提高空肠和回肠绒毛高度(P<0.05),显著降低回肠部位隐窝深度(P<0.05),极显著提高十二指肠、空肠和回肠绒毛高度/隐窝深度(P<0.01);显著降低空肠和回肠大肠杆菌数量(P<0.05),显著增加空肠和回肠乳酸杆菌数量(P<0.05)。结果提示,在仔猪感染致病性大肠杆菌K88时,饲粮添加枯草芽孢杆菌能有利于调节仔猪小肠发育和肠道微生物区系。

The study was conducted to study the effects of Bacillus subtilis（B.subtilis） on small intestine development and microflora of piglet administered with Escherichia coli（E.coli） K88.All eight castrated ＂Duroc ×Landrace × Yorkshire＂ male piglet used in this experiment had similar initial body weight（6.8±0.5） kg,they were divided into two groups（control group and test group） of 4 replicates of 1 pigs fed in single pen.Test group was fed the basal diet,and the test group was fed basal diet with 0.1% B.subtilis,and there were seven-day test period.All piglets were administered orally with E.coli K88.The result showed that piglet fed B.subtilis had greater villus height in jejunum and ileum（P〈0.05）,lower crypt depth（P〈0.05） in ileum,and greater ratio of villus height and crypt depth（P〈0.01） in duodenum,jejunum and ileum compared to piglets fed the unsupplemented diet.Feeding a Bacillus subtilis diet decreased the E.coli amount（P〈0.05） and increased the Lactobacillus amount（P〈0.05） in jejunum and ileum.The results indicated that feeding a Bacillus subtilis diet could modify the small intestine development and microflora.