Nav1.6在口腔黏膜白斑及口腔鳞癌中的表达

Expression of Nav1.6 in oral leukoplakia and oral squamous cell carcinoma

目的:探讨钠离子通道Nav1.6在口腔黏膜白斑及口腔鳞癌中的表达及意义。方法:采用免疫组化SP法及Western blot法检测Nav1.6在口腔正常组织(21例)、白斑(32例)及口腔鳞癌组织(63例)中的表达情况。结果:免疫组化检测,Nav1.6在正常组、口腔白斑组以及癌症组中的阳性表达率分别为6.25%、40%、76.74%;Nav1.6的表达与口腔鳞癌的病理分期有关。Western blot检测,正常组、口腔白斑组、癌症组中Nav1.6的蛋白表达量分别为:0.469±0.015、0.545±0.016、0.848±0.020(P<0.05)。结论:Nav1.6在口腔黏膜癌前病变及口腔鳞状细胞癌的发生和发展中可能起到了一定的作用。

Objective： To study the expression and significance of sodium channel Navl. 6 in oral leukoplakia and squamous cell car- cinoma. Methods： Immunohistochemistry and Western blot were used to detect the expression of Navl. 6 in 21 case of normal oral mucosa, 32 of oral leukoplakia and 63 of oral squamous cell carcinoma（OSCC）. Results： Immunohistochemical results showed that the positive rate of Navl. 6 in normal group, oral leukoplakia group and OSCC group was 6.25% , 40% and 76.74% respectively. The expression of Navl. 6 was related to the differentiation of oral squamous cell carcinoma. Western blot results revealed that the content of Navl. 6 in normal oral mucosa, oral leukoplakia and OSCC was 0. 469 ± 0. 015, 0. 545 ± 0. 016 and 0. 848± 0. 020 （ P 〈 0.05 ） respectively. Conclusion： Navl. 6 may play a role in the development of OSCC.