云南汉族人群HSPA1A基因多态性位点与肺癌的相关性研究

The Association of SNPs in HSPA1A Gene with Lung Cancer in Yunnan Han Population

目的：探讨HSPA1A基因多态性与肺癌发生发展的相关性。方法：选取云南地区汉族人群肺癌患者288例为病例组,健康体检人群298例为对照组,采用Taq Man探针基因分型方法对HSPA1A基因中的4个多态性位点（SNPs）位点rs12190359（C〉T）、rs562047（C〉G）、rs1008438（G〉T）及rs1043618（G〉C）进行基因分型,研究其等位基因、基因型及所构建的单倍型在两组人群中分布差异。结果：2组人群rs1043618（G〉C）等位基因C和G的分布频率差异有统计学意义（P=0.038）,其中等位基因C是肺癌发生的保护性因素[OR=0.767;95%CI（0.597～0.986）];rs12190359（C〉T）、rs562047（C〉G）和rs1008438（G〉T）的等位基因和基因型的分布差异无统计学意义（P〉0.05）;rs12190359与rs1008438（G〉T）以及rs562047（C〉G）与rs11043618（G〉C）的单倍型在2组间分布频率差异无统计学意义（P〉0.05）。结论：HSPA1A基因rs1043618（G〉C）等位基因C可能是云南汉族肺癌发生的保护性因素。

Objective： To explore the association of four single nucleotide polymorphisms （SNPs） rs12190359 C 〉 T, rs562047 C 〉 G, rs1008438 G 〉 T and rs1043618 G 〉 C in HSPA1A gene with the lung cancer in a Yunnan HaM population. Method： A total 288 patients with lung cancer and 298 healthy individuals were recruited in this study, and the genotyping of above-mentioned four SNPs was analyzed by Taqman assay. The distribution of alleles, genotypes and constructed haplotypes in the two groups was studied. Result： There were statistically significant differences in distribution frequency of alleles G and C of rs1043618 G 〉 C were significantly different between case and control groups （P =0.038）, and allel C was a protective factor of lung cancer[ OR =0. 767 ;95% CI （0. 597 - 0.986） ]. There were no statistically significant differences in distribution of the allele and genotype of rs12190359 C 〉 T, rs562047 C 〉 G and rs108438 G 〉 T between the case group and control group（P 〉 0.05 ）. There were no statistically significant differences in the distribution frequencies of haplotypes of rs12190359 and rs1008438 （ G 〉 T）, rs562047 （ C 〉 G） and rs11043618 （ G 〉 C） between case and control groups （P 〉0.05）. Conclusion： The C allele of rs1043618 G 〉 C in HSPA1A gene may be a protection factor of lung cancer, and the other sites may be not related to occurrence occurrence of lung cancer.