番茄红素对卵巢癌SKOV3细胞增殖和凋亡的影响

Effects of Lycopene on the Proliferation and Apoptosis of Ovarian SKOV3 Cancer Cell

目的研究番茄红素(lycopene,LP)对人卵巢癌SKOV3细胞增殖和凋亡的影响及其作用机制。方法取对数生长期SKOV3细胞随机分为5组:正常对照组、LP(20、10、5μg/ml)组和顺铂(40μg/ml)组(阳性对照组),每组10个复孔。给药干预48h后,观察比较各组细胞生长状况并采用MTT比色法测定细胞增殖抑制率;通过流式细胞术(flow cytometry)分析细胞周期的改变、检测细胞凋亡并计算细胞凋亡率,免疫印迹法(Western blot)检测caspase-3蛋白表达并进行半定量分析,反转录PCR(RT-PCR)技术检测Bax mRNA和bcl-2 mRNA表达并计算Bax/bcl-2表达比值。结果与正常对照组比较,LP各组SKOV3细胞呈现不同程度的细胞脱壁、细胞间松散等病理性状态,以LP 20μg/ml组最为显著;LP(20、10μg/ml)组细胞增殖抑制率显著升高,细胞周期被阻滞于G2/M期,细胞凋亡率显著升高,caspase-3蛋白表达量显著增高,bcl-2 mRNA表达显著下调、Bax mRNA表达显著上调,Bax/bcl-2表达比值显著升高,上述差异均具有统计学意义(P<0.05,P<0.01)。结论 LP具有抑制卵巢癌SKOV3细胞增殖并促进其凋亡的作用,作用机制可能与LP能够有效提高caspase-3蛋白表达并下调bcl-2 mRNA表达、上调Bax mRNA表达、提高Bax/bcl-2表达比值有关。

Objective To investigate the effects of lycopene（LP） on the proliferation and apoptosis of ovarian SKOV3 cancer cell. Methods The ovarian cancer SKOV3 ceils in logarithmic period were divided randomly into five groups： normal control group, LP（20, 10, 5μg/ml） groups and Cisplatin 40μg/ml group（positive control group）. 48h later, the cell morphology were observed, the cellular growth inhibition rate was calculated by MTT, cell cycle and apoptosis rate were analyzed by flow cytometry, the expression of caspase - 3 protein expression was detected by Western blot and were semi - quantitative analysised, and the expression of Bax mRNA and bcl - 2 mRNA were detected by RT - PCR. Results Compared with normal control group, the morphological of LP（20, 10μg/ml） groups were abnormalities, the cellular growth inhibition rate of LP（20, 10μg/ml） groups were significantly increased. The cell cycle was arrested at G2/M phase, the apoptosis rate was significantly increased. The expression of caspase -3 protein were significantly increased. The ex- pression of bcl- 2 mRNA was significantly decreased, while the expression of Bax mRNA was significantly increased, and the ratio of Bax/bcl - 2 were significantly increased. All of the difference above were significant （ P 〈 0.05, P 〈 0. 01 ）. Conclusion LP could effectively inhibit ovarian SKOV3 cancer cell proliferation and promote it apoptosis, which perhaps related to its effects of down regulating the expression of caspase - 3 and enhancing the ratio of bcl - 2/Bax.