都梁软胶囊对偏头痛模型大鼠中脑CGRP及CCK表达的影响

Effects of Duliang capsule on the expression of calcitonin gene-related peptide and cholecystokinin in the midbrain of a rat migraine model

目的研究都梁软胶囊对偏头痛模型大鼠中脑降钙素基因相关肽(CGRP)和缩胆囊肽(CCK)基因转录水平的影响,探讨其预防治疗偏头痛的作用机制。方法健康Wistar大鼠随机分4组,A为对照组,B为偏头痛组,C为都梁软胶囊对照组,D为都梁软胶囊治疗组。C、D组给予都梁软胶囊0.5g·kg^(-1)·d^(-1)灌胃,A、B组大鼠灌胃给予等量容积的Tween-80。灌胃7d后,将B、D两组大鼠造硝酸甘油型偏头痛模型,造模2h,保存中脑标本。实时荧光定量PCR检测CGRP和CCK的转录水平。结果 D组大鼠中脑CGRP转录水平明显低于B组(0.64±0.35和1.61±0.51,P<0.05)。C组大鼠中脑CCK转录水平明显低于A组(0.32±0.31和1.21±0.38,P<0.05)。结论都梁软胶囊可以干预中脑CGRP和CCK的表达,从而影响内源性痛觉调制系统的功能。

Objective To study the effects of Duliang capsule on the expression of calcitonin gene‐related peptide（CGRP） and cholecystokinin（CCK） in the midbrain of a rat migraine model ,and explored treatment effects and mechanisms of Duliang cap‐sule on rats with migraine .Methods A total of 24 rats were randomly divided into four groups ：normal control groups（A） ,migraine model groups（B） ,Duliang capsule control groups（C） and Duliang capsule treatment groups（D） .C and D were intragastrically per‐fused with Duliang capsule（0 .5 g · kg -1 ·d-1 ） .After 7 days ,nitroglycerin was subcutaneously injected into the buttocks of the B and D to induce migraine .Two hours after nitroglycerin injection ,the midbrain were isolated CGRP and CCK expression in midbrain were determined using SYBR Green I real‐time quantitative PCR .Results CGRP mRNA expression was significantly lower in mid‐brains of rats in the Duliang capsule treatment groups compared with migraine model groups（P〈0 .05） .CCK mRNA expression was significantly lower in midbrains of rats in the Duliang capsule control groups compared with normal control groups（P〈0 .05） . Conclusion Duliang capsule can exhibit the expression of CGRP and CCK in the midbrain of the migraine rats .weaken the CGRP and CCK‐induced inhibition of the analgesic effects of opioid peptides .