脂联素后处理对大鼠心肌缺血-再灌注损伤的保护作用

The role of adiponectin postconditioning on myocardial ischemia reperfusion injury in rats

目的探讨脂联素(ADP)后处理对大鼠心肌缺血-再灌注损伤(MIRI)是否有保护作用。方法 SD大鼠随机分为:(1)假手术组(Sham组),冠状动脉左前降支(LAD)仅穿线不断流;(2)心肌缺血-再灌注损伤组(MIRI组),LAD结扎30min后再灌注120min;(3)脂联素后处理组(ADP组),LAD再灌注前20 min注射ADP,其余同MIRI组。检测各组血浆乳酸脱氢酶(LDH)、心肌肌钙蛋白I(cTnI)和丙二醛(MDA)的水平,检测心肌超氧化物歧化酶(SOD)和MDA的水平,各组心肌HE染色并描记心电图。结果MIRI组血浆LDH(735.21±110.92)U/L、cTnI(37.96±5.97)μg/mL和MDA(4.55±0.18)nmol/L较Sham组升高(P<0.05),ADP组血浆LDH(579.25±69.01)U/L、cTnI(21.07±5.77)μg/mL和MDA(2.99±0.22)U/L水平较MIRI组降低(P<0.05);与Sham组相比,MIRI组心肌SOD水平(11.47±1.67)U/mg降低而MDA水平(7.99±0.59)nmol/mg升高(P<0.05);与MIRI组相比,ADP组心肌SOD水平(13.93±0.97)U/mg升高而MDA水平(5.74±0.64)nmol/mg降低(P<0.05)。结论 ADP后处理可以减轻大鼠MIRI,可能与减少脂质过氧化和增强自由基清除有关。

Objective To study the role of adiponectin （ADP） postconditioning on myocardial ischemia reperfusion injury （MIRI） in rats .Methods SD rats were divided randomly into 3 groups ：⑴Group Sham were processed identically except the left anterior descending coronary artery（LAD） and was not ligated;⑵ Group MIRI were subjected to LAD occlusion for 30 min and reperfusion for 120 min;⑶ Group ADP were subjected to ADP injection before reperfusion .The titer of lactate dehydrogenase （LDH） ,cardiac troponin I（cTnI） and malondialdehyde（MDA）in plasma were observed .The levels of superoxide dismutase（SOD） and malondialdehyde（MDA）in myocardial tissue were observed .The results of HE staining and electrocardiograms（ECG） was ob‐served .Results Compared with Group Sham ,LDH ,cTnI and MDA in plasma in Group MIRI increased （P〈0 .05）;Compared with Group MIRI ,LDH ,cTnI and MDA in plasma in Group ADP declined （P〈0 .05）;Compared with Group Sham ,SOD decreased and MDA increased in myocardial tissue in Group MIRI （P〈0 .05）;Compared with Group MIRI ,SOD increased and MDA decreased in myocardial tissue in Group ADP （P〈0 .05） .The myocardial injury was serious in Group MIRI while it was lightened in Group ADP .There were acute ischemia ECG change in Group MIRI while it was slight in Group ADP .Conclusion ADP postconditioning is a protective factor against MIRI in rats ,it is related to eliminating free radicals and alleviating the damage of lipid peroxidation .