高效液相色谱法测定灯盏细辛提取物中两种有效成分的含量

The High Performance Liquid Chromatography in the determination of the content of two kinds of active ingredients in Erigeron breviscapus extract

目的：探讨灯盏细辛提取物中野黄芩苷、3,4-二-O-咖啡酰奎宁酸2种有效成分含量的高效液相色谱测定方法。方法：色谱柱选择Dikma Kromasil C18（250mm×4.6mm,5.0μm）,流动相选择乙腈：0.2%磷酸溶液=30∶70,检测波长为332nm,柱温为35℃。结果：野黄芩苷的线性回归方程为Y=1.554×103-0.201×102（r=0.9997）;3,4-二-O-咖啡酰奎宁酸的线性回归方程为Y=1.724×103-0.081×102（r=0.9996）;野黄芩苷在0.0341~1.3121μg范围内,3,4-二-O-咖啡酰奎宁酸在0.0592~2.3426μg范围内呈良好的线性关系。结论：采用高效液相色谱法同时测定灯盏细辛提取物中2种有效成分的含量,操作简单,精密度高,重现性好,可以作为灯盏细辛提取物中这2种有效成分的定量分析方法。

Objective：To investigate the High Performance Liquid Chromatography in the determination of the content o Scutellarin and Isochlorogenic acid B f two kinds of active ingredients in Erigeron breviscapus extract. Methods：The Column Selection was Dikma Kromasil C18（250mm×4.6mm,5.0μm）,the mobile phase was acetonitrile：0.2% phosphoric acid solution=30∶70,the detection wavelength was 332 nm,the column temperature was 35℃. Results：The scutellarin linear regression equation was：Y=1.554×103-0.201×102（r=0.9997）; Isochlorogenic acid B regression equation was：Y=1.724×103-0.081×102（r=0.9996）; scutellarin was at the range of 0.0341~1.3121μg,3,4-O-dicaffeoylquinic range was 0.0592~2.3426μg,which showed a good linear relationship. Conclusion：The HPLC method for the determination of the content of two kinds of active ingredients in Erigeron breviscapus extract is simple and easy operation,with high precision,reproducibility,and can be used as a quantitative analysis method for the two kinds of active ingredients in Erigeron breviscapus extract.