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食管鳞癌细胞中ESCCAL_1对蛋白激酶磷酸化的调节作用

Regulatory effects of ESCCAL_1 on protein kinase phosphorylation in esophageal squamous cell carcinoma
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摘要 目的探讨ESCCAL_1在食管鳞癌中发挥功能的可能机制。方法使用人磷酸化激酶阵列试剂盒检测ESCCAL_1敲减前后EC9706细胞中相关蛋白激酶磷酸化水平,对各蛋白条带进行灰度值定量分析。结果 KD组与NC组中FAK、JNK1、Src、AMPKα1等蛋白激酶的相对磷酸化水平比值分别为2.76∶1、2.20∶1、2.15∶1、2.01∶1,表达量明显降低(P<0.05)。结论 ESCCAL_1可能通过调节相关蛋白激酶的磷酸化水平调控EC9706细胞生长。 Objective To explore the possible mechanism of ESCCAL_I in esophageal squamous cell carcinoma. Methods Human phospho - kinase array kit was used to detect the phosphorylation of protein kinase in EC9706 cells transfected with ESC- CAL_I - shRNA or control. Each gray value of protein band was quantitatively analyzed. Results The relative gray value ratios of FAK,pJNK1 ,pSrc,pAMPKcd between KD group and NC group were 2.76：1, 2. 20：1, 2. 15：1, 2. 01：I respectively, and the phosphorylation of these protein kinases in KD group was significantly down - regulated （P 〈 0. 05）. Conclusion ESCCAL_I may regulate the EC9706 cell proliferation and metastasis through protein kinase phosphorylation.
作者 曹新广 崔渊博 陈小兵 曹巍
机构地区 郑州大学附属肿瘤医院普外科 郑州市中心医院转化医学中心
出处 《河南医学研究》 CAS 2016年第10期1731-1733,共3页 Henan Medical Research
关键词 食管鳞状细胞癌 ESCCAL_1 蛋白激酶磷酸化 esophageal squamous cancer ESCCAL_1 protein kinase phosphorylation
分类号 R735.1 [医药卫生—肿瘤]
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河南医学研究
2016年 第10期

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