发酵前后天龙粉水溶性活性物质含量对比研究

Content of water-soluble active substances in Tianlong powder before and after fermentation: a comparative analysis

目的:比较中药天龙粉发酵前后水溶性活性物质的含量。方法:采用超声水提取法分别提取天龙粉发酵前后所含的水溶性活性成分,通过改良Lowry法测定其蛋白质含量、苯酚-硫酸法测定其多糖含量、定磷法测定其核酸含量。结果:天龙粉发酵前蛋白含量为68.5730mg/g,发酵后为130.6559mg/g;天龙粉发酵前多糖含量为6.5771mg/g,发酵后为13.4492mg/g;而天龙粉发酵前核酸含量为17.7146mg/g,发酵后为9.1373mg/g。结论:天龙粉发酵前的核酸含量较高,而发酵后的蛋白质和多糖含量较高。

Objective： To investigate the content of water-soluble active substances in Tianlong powder before and after treatment through a comparative analysis. Methods： Ultrasonic water extraction was used to extract water-soluble active substances in Tianlong powder before and after fermentation. The modified Lowry method was used to measure the content of protein,the phenol-sulfuric acid method was used to measure the content of polysaccharides,and the method of phosphorus determination was used to measure the content of nucleic acid. Results： The content of protein in Tianlong powder was 68. 5730 mg / g before fermentation and 130. 6559 mg / g after fermentation. The content of polysaccharides was 6. 5771 mg / g before fermentation and 13. 4492 mg / g after fermentation.The content of nucleic acid was 17. 7146 mg / g before fermentation and 9. 1373 mg / g after fermentation. Conclusion： Tianlong powder has a higher content of nucleic acid before fermentation and higher content of protein and polysaccharides after fermentation.