大天鹅肾组织显微结构及细胞凋亡相关蛋白的表达

Histological Microstructure and Expression of Apoptosis-related Protiens in Whooper Swan(Cygnus cygnus) Kidney

为了解大天鹅(Cygnus cygnus)肾的结构特点和相关活性蛋白的表达情况,采用石蜡切片、H.E染色和免疫组化法,观察分析大天鹅的肾组织显微结构和Caspase-3、Bcl-2、Bax及AQP-3的表达。大天鹅的肾组织主要由肾单位、集合管和结缔组织构成;肾小球由一团盘曲的毛细血管构成,近端小管由单层立方上皮组成,游离面有刷状缘,细段由单层扁平上皮组成,远端小管由立方形上皮组成,腔面无刷状缘。近端小管上皮细胞中有Caspase-3、Bcl-2、Bax阳性表达,远端小管上皮细胞有Caspase-3阳性表达,集合管上皮细胞有Bcl-2、AQP-3阳性表达,在肾小球毛细血管内皮有Bcl-2阳性表达。Caspase-3、Bcl-2、Bax及AQP-3共同调节细胞的生长与凋亡过程,可能在稳定鸟类肾单位和集合管结构及调节肾组织水平衡等方面有重要的作用。

In order to explore microstructure and expression of apoptosis-related protiens in Whooper Swan （Cygnus cygnus） kidney, paraffin sections of kidney were observed after H.E staining and expressions of Caspase-3, Bcl-2, Bax, and AQP-3 proteins were examined by immune histochemical methods. The results show that the kidney tissue of Whooper Swan consists of nephrons, collecting ducts and connective tissue. The glomerulus of renal corpuscle was composed of convoluted capillaries. The proximal tubule was lined with simple cuboidal epithelium, with a well-developed brush border. The thin limb and the distal tubule with brushless border respectively consist of simple squamous epithelium and cubic epithelium （Fig. la, b）.Caspase-3, Bcl-2 and Bax were expressed in the epithelium of the proximal tubule. Caspase-3 was present in the distal tubule. Bcl-2 and AQP-3 were expressed in the collecting ducts. Bcl-2 was expressed occasionally in the endothelium of glomerular capillary （Fig. lc - i）. Caspase-3, Bcl-2, Bax and AQP-3 may have an important role in stabilizing the structure of nephron and collecting duct and the regulation of water balance in Whooper Swan kidney.