摘要
以哈密大枣无菌苗叶片为试材,以根癌农杆菌LBA4404为媒介将抗寒基因转录因子CBF基因导入哈密大枣,利用PCR、RT-PCR对转化植株进行鉴定,建立了哈密大枣的遗传转化体系。结果表明:在对哈密大枣无菌苗叶片进行遗传转化时,预培养2d,农杆菌菌液OD600为0.5,浸染20min,共培养3d的转化效率为最高。试验共获得7个抗性再生转化系。经PCR鉴定,CBF基因在7个抗性再生转化系中均为阳性;经RT-PCR分析,CBF基因在7个抗性再生转化系中均得到表达。
Hami Zizyphus jujubaleaves were used as test materials to study genetic transformation of Hami Zizyphus jujuba by using CBFtranscription factor of antifreeze gene,the CBFtranscription factor of antifreeze gene was induced by using Agrobacterium tumefactions LBA4404,the transgenic plants were identified by using PCR and RT-PCR.The results showed that the highest transformation efficiency was obtained when leaf explants were cultured 3days after precultured for 2days and infested for 20 minutes with Agrobacterium tumefactions（OD600=0.5）.7resistance regenerated transformation systems were obtained in this experiment.CBFgenes were positive by using PCR identify and expressed by using RT-PCR analysis in 7resistance regenerated transformation systems.
作者
高启明
王斌
赛买提.吐尔逊
徐明
罗淑萍
GAO Qiming WANG Bin Saimaiti · TUERXUN XU Ming LUO Shuping(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou, Henan 450009 Technology Extension Cemer of Hami Prefecture Forestry and Fruit Industry, Hami, Xinjiang 839000 College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang 830052)
出处
《北方园艺》
CAS
北大核心
2016年第18期94-98,共5页
Northern Horticulture
基金
新疆维吾尔自治区高技术研究发展资助项目(201211108)
关键词
哈密大枣
CBF基因
农杆菌介导
Hami Zizyphus jujuba
CBF gene
Agrobacterium-mediated
