QuEChERS-超高效液相色谱—串联质谱法在食品真菌毒素检测中应用的研究进展

Research advances on application of QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry in mycotoxin analysis in foods

真菌毒素是一类由产毒丝状真菌产生的有毒次生代谢产物,能诱发人畜各种生理损害,是世界各地食品和农产品的主要污染物之一。建立高效的真菌毒素分析方法将有效降低真菌毒素的暴露风险,复杂基质中多种真菌毒素的联合提取净化和检测是建立这类分析技术的前提保证。Qu ECh ERS(quick,easy,cheap,effective,rugged and safe)方法具有简单、快速、回收率高、试剂用量少、安全等优点,适用于大批量样品的检测。超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)可同时用于定性和定量分析,具有检出限低、灵敏度高等优点。本文根据Qu ECh ERS前处理技术的特点,结合样品真菌毒素的理化性质和污染特点,对Qu ECh ERS-UPLC-MS/MS技术在真菌毒素分析中的应用做了简要综述。

Mycotoxins are the secondary metabolites produced by certain filamentous fungi, which can induce physiological damage on animals and humans, and they are regarded as one of the main pollution source in foods and agriculture products all over the world. Simultaneous extraction of mycotoxins from complex sample matrices is a prerequisite to establish a high-throughput analytical method, which in turn will effectively reduce the mycotoxins exposure risk. Qu ECh ERS is a sample preparation technique with the advantages of being simple and rapid, high recovery rate, low solvent consumption and safety, which is suitable for the detection of large beach samples. Ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） can be used for both qualitative and quantitative analysis with the advantages of low detection limit and high sensitivity. This paper summarized the application of Qu ECh ERS-UPLC-MS/MS techniques in the analysis of mycotoxins, which combined with the characteristics of Qu ECh ERS method technology and the physicochemical properties and pollution characteristics of mycotoxins.