胶体金技术快速测定葡萄酒中赭曲霉毒素A

Rapid detection of ochratoxin A in wines by gold immunochromatography assay

目的利用胶体金免疫层析技术建立葡萄酒中赭曲霉毒素A的快速筛查方法。方法样品经过层析、孵育,通过i Check-单卡单测快检仪的图像分析进行定量检测,同时对进口与市售国产葡萄酒进行筛查。结果赭曲霉毒素A在添加水平为2.0、5.0μg/L时,红葡萄酒的平均回收率分别为92.5%、72.0%;相对标准偏差(RSD,n=6)分别为5.84%、2.59%;桃红葡萄酒的平均回收率分别为87.0%、76.6%;相对标准偏差(RSD,n=6)分别为7.70%、5.56%;白葡萄酒的平均回收率分别为84.0%、78.4%;相对标准偏差(RSD,n=6)分别为5.67%、3.54%。该方法定量限为1.0μg/L。同时与酶联免疫方法和液相色谱方法比较,通过t检验表明胶体金法不存在系统误差。市售葡萄酒赭曲霉毒素A的含量低于欧盟的限量标准(2.0μg/L)。结论胶体金的方法具有快捷、灵敏、特异性等特点,适合于大批量葡萄酒样品的现场快速筛查。

Objective To establish a rapid method for the detection of ochratoxin A（OTA） in wines by gold immunochromatography assay（GICA）. Methods After chromatography and incubation, the samples were quantitatively detected by i Check-single card single measuring detector, meanwhile some of imported and domestic wines were screened. Results The recoveries of OTA spiked at 2 levels of 2.0 and 5.0 μg /L in red wine were 92.5% and 72.0% respectively with the relative standard deviations（RSDs） of 5.84% and 2.59%（n=6）; in pink wine were 87.0% and 76.6% respectively with the RSDs of 7.70% and 5.56%（n=6）; and in white wine were 84.0% and 78.4% respectively with the RSDs of 5.67% and 3.54%（n=6）. The quantification limit of GICA was 1.0 μg /L. Compared with enzyme-linked immunosorbent assay（ELISA） and high performance liquid chromatography（HPLC）, the results of t-test demonstrated that there was no systematic error in GICA. The results of market wines met the requirements of European Union limit standard（2.0 μg /L）. Conclusion The GICA method is rapid, sensitive and specific, which is suitable for preliminarily on-site screening of OTA in the samples of great batch rapidly.