微切变—助剂互作技术辅助提取柑橘皮总黄酮的工艺研究

Optimization of total flavonoids extraction from citrus peel by press-shear assisted interaction technology

目的研究以氯化钾为助剂的微切变—助剂互作技术辅助提取柑橘皮中总黄酮的最佳工艺条件。方法以柑橘皮为主要原料,氯化钾为助剂,乙醇为提取溶剂。采用单因素试验和正交试验,以柑橘皮中总黄酮的提取率为考察指标,对助剂添加量、研磨时间、乙醇浓度和料液比等4个因素进行研究,优化了微切变—助剂互作技术辅助提取柑橘皮总黄酮的最佳工艺条件。结果在最佳工艺条件即助剂氯化钾添加量为4%(W:W),研磨时间为35 min,乙醇浓度为40%(V:V),料液比为1:50(m:V)的条件下,总黄酮提取率为2.87%,比传统的热回流法提高了54.30%。结论微切变—助剂互作技术具有提取率高、提取周期短、乙醇用量少、无需加热等特点,是提取柑橘皮总黄酮的一种有效方法。

Objective To optimize the extraction conditions of total flavonoids from citrus peel using press-shear assisted interaction technology with potassium chloride as additive. Methods The study used citrus peel as main raw materials, potassium chloride as additive, and ethanol as the organic solvent. The optimum extraction parameters including the dosage of sodium chloride, grinding time, ethanol concentration and ratio of material to solvent for extracting total flavonoids from citrus peel were investigated by one-factor experiment and orthogonal design, and the extraction yield of total flavonoids in citrus peel was regarded as examining index. Results The highest extraction efficiency of total flavonoids was up to 2.87% when the dosage of potassium chloride was 4%（W：W）, grinding time was 35 min, ethanol concentration was 40%（V：V） and the ratio of material to solvent was 1：50（m：V）. Under the optimum conditions, the extraction yield of total flavonoids was increased by 54.30% than the traditional hot reflux method. Conclusion The press-shear assisted interaction technology has the advantages of high extraction rate, short extraction duration, low consumption of ethanol and no heat, which is an effective method for total flavonoids extraction from citrus peel.