微切变—助剂互作技术制备柑橘皮粉的保健功能研究

Health functions of citrus peel powder processed by press-shear assisted interaction technology

目的考察微切变-助剂互作技术制备的柑橘皮粉的保健功能,以促进柑橘皮的合理利用。方法将健康昆明小鼠随机分为空白对照组、粗粉组和微切助粉组,每组10只,空白对照组小鼠饲喂商品鼠粮,实验组小鼠分别饲喂含柑橘皮粗粉或经微切变-助剂互作技术处理柑橘皮制备的微切助粉鼠粮。连续饲养小鼠一定周期后,研究柑橘皮微切助粉对小鼠多种保健功能的影响。结果与对照组相比,柑橘皮微切助粉极显著地增加小鼠的体重,免疫器官指数,血清中抗体Ig G、Ig A和Ig M的含量和血清中SOD的水平,说明柑橘皮可提高小鼠免疫力。二甲苯所致小鼠耳肿胀试验证明柑橘皮微切助粉的耳肿胀抑制率为74.3%,且抗炎效果接近阿司匹林,表明柑橘皮具有非常好的抗炎作用。另外,柑橘皮微切助粉组小鼠的负重游泳时间(6.88±0.27 min)是对照组(4.67±0.18 min)的1.47倍,表明柑橘皮具有抗疲劳作用。与对照组相比,柑橘皮微切助粉极显著地缩短了戊巴比妥钠所致小鼠的入睡时间和延长了睡眠持续时间,表明柑橘皮具有镇静催眠作用。结论柑橘皮具有提高小鼠机体免疫力、抗炎、抗疲劳、镇静催眠的作用,且柑橘皮微切助粉的各种保健效果优于其粗粉。

Objective To investigate the the health functions of citrus peel powder processed by press-shear assisted interaction technology（PAI）, so as to improve the rational use of citrus peel. Methods Kunming mice were randomly divided into blank control group, coarse powder group and PAI powder group with ten mice in each group. Animals in blank control group were fed with standard mouse chow. Animals in experimental groups were fed with mouse chow containing citrus peel coarse powder or PAI powder, respectively. After a certain period of continuous feeding, several health functions of citrus peel PAI powder were investigated in mice. Results Compared with control group, the body weight, immune organ indexes, serum antibodies content and SOD value of mice in experimental group were significantly increased, implying that citrus peel had immunomodulatory effect in mice. It was demonstrated that inhibition rate of xylene-induced ear swelling of mice in experimental group was 74.3% and the anti-inflammatory effect was close to aspirin, showing citrus peel had significant anti-inflammatory effect. Moreover, loaded-swimming time of mice in citrus peel PAI powder group（6.88±0.27 min） was 1.47 times of that of control group（4.67±0.18 min）, indicating citrus peel had anti-fatigue effect. Compared with control group, citrus peel PAI powder had significantly shortened the time to fall asleep and prolonged sleep duration in mice induced by pentobarbital sodium, suggesting that citrus peel had sedative and hypnotic effects. Conclusion Citrus peel has many pharmacological functions, including immunomodulatory, anti-inflammatory, anti-fatigue, sedative and hypnotic effects, and the various health effects of citrus peel PAI powder are superior to its coarse powder.