鸡肉中金刚烷胺和利巴韦林残留基体标准物质的研制

Preparation of matrix reference material of amantadine and ribavirin residues in chicken muscle

目的建立含金刚烷胺和利巴韦林多残留鸡肉糜样自然基体标准样品的研制和定值方法。方法通过添加金刚烷胺和利巴韦林饲养白羽肉鸡获得阳性样本,然后经过取样、均质、包装、辐照灭菌、均匀性检验、稳定性检验、协同定值等过程,制备预期目标含量水平的含金刚烷胺和利巴韦林的多残留鸡肉糜样自然基体标准样品。结果经10家实验室采用液相色谱-串联质谱法进行协同定值,金刚烷胺和利巴韦林的特性值分别为10.1±1.0μg/kg(k=2),11.3±1.6μg/kg(k=2)。结论研究表明获得的样品均匀性良好,稳定性达到10个月,能够用于兽药残留检测的质量控制。

Objective To establish a method for preparation and certification the matrix reference material（RM） of amantadine and ribavirin residues in chicken muscle. Methods The reference material was prepared by adding amantadine and ribavirin to feed white-feather chicken. The reference material minced chicken muscle with expected two antivirals（containing amantadine and ribavirin） residues was developed by the procedure of sampling, homogeneous, sealed packaging, irradiation sterilization, homogeneous testing and stability testing. Results The concentrations of the chemical constituents of the samples were certified through the collaborative analysis program participated by 10 laboratories with liquid chromatography-tandem mass spectrometry（LC-MS/MS） method, and the uncertainty assessment was performed. The property values and expanded uncertainties of amantadine and ribavirin were 10.1±1.0 μg/kg（k=2） and 11.3±1.6 μg/kg（k=2）, respectively. Conclusion The results showed that the uniformity of samples was good, and the expiration date of reference material was at least 10 months. The reference material can be used in the quality control of residues detection.