基于HPLC与NIRS指纹图谱分析不同形态的乌药根

Analysis of different forms Linderae Radix based on HPLC and NIRS fingerprints

为了分析鉴别3种不同形态的乌药根,采用高效液相色谱和近红外光谱指纹图谱相结合的方法进行综合评价。将乌药纺锤根、直根和老根进行分组,建立其高效液相色谱(HPLC)指纹图谱,并使用SPSS软件进行聚类分析;采集乌药近红外光谱(NIRS)图,运用判别分析法,建立乌药最佳定性分析模型。结果显示,乌药纺锤根和乌药直根相似度≥0.990,乌药老根相似度≤0.85;聚类分析和NIRS判别分析模型将乌药3个不同形态根清晰地聚为两类,界限明显。说明乌药老根与纺锤根、直根有很大的品质差异。HPLC和NIRS指纹图谱2种方法结合,能够快速准确地用于不同形态乌药根的鉴别。

Three different forms of Linderae Radix were evaluated by HPLC combined with NIRS fingerprint. The Linderae Radix was divided into three forms,including spindle root,straight root and old root. The HPLC fingerprints were developed,and then cluster analysis was performed using the SPSS software. The near-infrared spectra of Linderae Radix was collected,and then established the discriminant analysis model. The similarity values of the spindle root and straight root all were above 0. 990,while the similarity value of the old root was less than 0. 850. Two forms of Linderae Radix were obviously divided into three parts by the NIRS model and Cluster analysis. The results of HPLC and FT-NIR analysis showed the quality of Linderae Radix old root was different from the spindle root and straight root. The combined use of the two methods could identify different forms of Linderae Radix quickly and accurately.