地榆鞣质与皂苷组分配伍对儿茶素、表儿茶素和地榆皂苷Ⅰ在大鼠体内药动学参数的影响

Effects of sanguisorba tannins and saponins compatibility on pharmacokinetic parameters of catechin,epicatechin and ziyuglycoside Ⅰ in rats

采用药代动力学相关技术和方法,研究地榆鞣质和皂苷不同比例配伍[鞣质-皂苷(1∶1)和鞣质-皂苷(8∶1)]大鼠灌胃(50 mg·kg-1)给药后,儿茶素、表儿茶素和地榆皂苷Ⅰ在大鼠体内的药动学行为的变化。并采用Kinetica 5.0软件计算药动学参数。结果显示,所建分析检测方法的专属性、线性、回收率、精密度及稳定性均符合试验要求;地榆鞣质与皂苷1∶1配伍时,儿茶素和表儿茶素Vd和CL显著增大(P<0.05),MRT显著缩短(P<0.05),Cmax和AUC显著减小(P<0.05)。而8∶1配伍时,儿茶素和表儿茶素的Vd和CL显著减小(P<0.05),MRT显著延长(P<0.05),Cmax和AUC显著增大(P<0.05);而地榆鞣质与皂苷配伍且随着鞣制比例的增加,地榆皂苷Ⅰ在大鼠体内的Cmax和AUC显著增大(P<0.05),Vd和CL显著减小(P<0.05),Tmax明显滞后,MRT也显著延长(P<0.05)。结果表明,地榆鞣质与皂苷组分8∶1配伍时儿茶素、表儿茶素和地榆皂苷Ⅰ在大鼠体内均表现出良好药动学行为,可作为地榆鞣质和地榆皂苷组分配伍比例的参考。

To study the effects of sanguisorba tannins and saponins compatibility at different proportions [tannins-saponins（ 1∶ 1）and tannins-saponins（ 8∶ 1） ] after intragastric administration（ 50 mg·kg-1） on pharmacokinetic parameters of catechin,epicatechin and ziyuglycoside Ⅰ in rats by using pharmacokinetic techniques and methods. Kinetica 5. 0 software was used to calculate the pharmacokinetic parameters. The results showed that the specificity,linearity,recovery rate,precision and stability of the established detection method were in line with the test requirements. When the sanguisorba tannins and eaponins were combined at the rate of 1∶ 1,Vd and CL of catechin and epicatechin were increased significantly（ P〈0. 05）; MRT was significantly shortened（ P〈0. 05）; Cmaxand AUC were significantly reduced（ P〈0. 05）. When the sanguisorba tannins and saponins were combined at the rate of 8∶ 1,Vdand CL of catechin and epicatechin were significantly reduced（ P〈0. 05）; MRT was significantly prolonged（ P〈0. 05）; Cmaxand AUC were increased significantly（ P〈0. 05）. In addition,with the increase in proportion of sanguisorba tannins in the compatibility,Cmaxand AUC of ziyuglycoside Ⅰ were increased significantly（ P〈0. 05）; Vdand CL were significantly reduced（ P〈0. 05）,Tmaxwas obviously lagging behind,and MRT was also significantly prolonged（ P〈0. 05）. In our present study,catechin,epicatechin and ziyuglycosideⅠ showed good pharmacokinetic behavior in rats when sanguisorba tannins and saponins were combined at the rate of 8∶ 1 in compatibility,which could be used as a reference for the proportion in sanguisorba tannins and saponins compatibility.