摘要
利用rDNA ITS和叶绿体trnL-F序列,对17份西南地区冰球子进行分子鉴定。采用改进CTAB法提取独蒜兰叶片基因组DNA,利用通用引物ITS 1和ITS 4以及引物trnL-F对其DNA中rDNA ITS区和叶绿体trnL-F序列进行扩增,对目的片段进行测序并对测序结果进行聚类分析。结果表明,17份西南独蒜兰代表3个种,即云南独蒜兰、独蒜兰和一未定种,药源基本可靠。
The rDNA ITS and chloroplast trnL-F sequences were applied to molecularly identify 17 pleione in southwest China.The genomic DNA of pleione was extract from leaves with the improved CTAB method.Then, the rDNA ITS and chloroplast trnL-F in the DNA were amplified with universal primers ITS 1 and ITS 4 and primer trnL-F separately.The expected fragments were sent to sequece and the results for cluster analysis showed that 17 pleione represent three species, Pleione yunnanensis,P.bulbocodioides and an unknown species, suggesting that the medicinal resouces of Bingqiuzi in Southwest China was basicly reliable.
作者
朱英
杨友联
黄永会
刘永翔
张丽娜
刘作易
ZHU Ying YANG Youlian HUANG Yonghui LIU Yongxiang ZHANG Lina LIU Zuoyi(Guizhou.Institute oI Biotechnology, Guiyang 550006, China The Key Laboratory for Agri. Biotechnology of Guizhou,Guiyang 550006,China Department of Life Science, Liupanshui Normal College, Shuicheng 553006 ,China Guizhou Institute of Modern Chinese Medicinal Materials,Guiyang 550006,China Guizhou Academy of Agri.Sci, Guiyang 550006, China)
出处
《种子》
北大核心
2016年第9期12-14,19,共4页
Seed
基金
贵州省中药现代化科技产业研究开发专项项目(编号:黔科合社字[2009]5036号)
贵州省中药现代化科技产业研究开发专项项目(编号:黔科合中药字[2011]5039号)
贵州省科研机构创新能力建设专项(编号:黔科合院所创能[2010]4002)
贵州省农业科学院资助项目(编号:黔农科合(创新基金)2012001号)
贵州省农业科学院基金项目(编号:黔农科合(基金)2011021)
