聚乳酸可吸收根管桩膜生物相容性实验

Biocompatibility of polylactic acid-absorbable root post film

目的检测通过溶剂挥发成膜法制备的聚乳酸可吸收根管桩膜的生物相容性。方法采用溶剂挥发成膜法制备聚乳酸根管桩膜,进而制备聚乳酸桩膜浸提液,检测聚乳酸桩膜的生物相容性。体外培养人牙龈成纤维细胞(HGF),用聚乳酸桩膜浸提液培养HGF,采用形态学观察法、四甲基偶氮唑盐(MTT)法、流式细胞术检测HGF的形态学变化、细胞毒性反应及活细胞率。将HGF接种于聚乳酸桩膜上,通过4’,6-二脒基-2-苯基吲哚(DAPI)染色观察HGF在聚乳酸桩膜上的生长情况。将聚乳酸桩膜植入到SD大鼠皮下,于第1、4、8、12周从动物背部取出植入材料及周围皮下组织,观察切口大体表现,并对组织行苏木精-伊红染色,通过观测炎症细胞浸润程度检验材料对局部组织炎症和异物反应的情况。结果聚乳酸桩膜浸提液对HGF的增殖无明显影响,无明显细胞毒性;HGF可在聚乳酸桩膜上黏附和生长。皮下植入实验:实验组标本的大体观察和苏木精-伊红染色观察结果均与对照组相似,符合生物安全性要求。结论通过溶剂挥发成膜法制备的聚乳酸可吸收根管桩膜具有良好的生物相容性,可用于进一步临床研究。

Objective To study the biocompatibility of the polylactic acid （PLA）-absorbable root post film prepared by solvent evaporation film. Methods The PLA post film-leaching liquor was prepared by the solvent evaporation of PLA root post films, and its biocompatibility was measured. Sources of human gingival fibroblasts （HGF） were cultivated in vitro and identified initially by the immunohistochemistry method. The toxicity reaction, survival rate, and morphological change of HGF incubated in PLA post film-leaching liquor were observed and tested by morphological observation, 3-（4,5-dimethylthiazol- yl）-2,5-diphenyltetrazolium bromide （MTT） assay, and flow cytometry. The HGF were implanted on the PLA post film and observed by 4＇,6-diamidino-2-phenylindole staining. After implanting the PLA post film, the surrounding subcutaneous tissue of SD rats and the implants were removed from the back of the rats in the 1st, 4th, 8th, and 12th weeks to observe the general condition of the incisions. The organization was given the hematoxylin-eosin （HE） stain to find the organization condition of local tissue inflammation and foreign body reaction through the infiltration degree of inflammatory fibroblasts. Results The effects of the PLA post film-leaching liquor on the proliferation of HGF and fibroblast toxicity were insignificant. The living fibroblast rate was similar to the normal control group. The HGF were able to grow on the PLA film. The results of the orga- nization general observation and HE staining of the rats were similar to the results for the control group, which met all the demands made by biological safety. Conclusion The PLA-absorbable root post film prepared by the solvent evaporation film has good biocompatibility and can be used for further clinical research.