胆红素在哮喘模型建立过程中的保护作用

The Protective Effect of indirect Bilirubin in the process of Asthmatic Modeling

探讨胆红素在哮喘动物模型建立过程中能否发挥保护作用。通过用卵清蛋白(OVA)对BALB/C小鼠进行致敏、激发建立哮喘模型,并在致敏和激发阶段给予间接胆红素干预。造模成功后通过肺组织病理切片及支气管肺泡灌洗液炎症细胞分类计数观察哮喘模型小鼠的气道炎症程度,ELISA方法测定血清中OVA-sIgE及相关细胞因子IL-4、IFN-γ、IL-2、IL-17、IL-10和TGF-β的水平,免疫组化法检测肺组织IL-17的表达,并检测氧化应激相关指标。研究发现,经胆红素干预后小鼠体内间接胆红素水平升高,肺组织炎症细胞浸润减少,OVA-sIgE和IL-4水平较低而IFN-γ、IL-2和IL-10水平较高,IFN-γ/IL-4增高;肺组织IL-17表达较低,氧化应激反应降低,而对TGF-β的影响较小。可见胆红素在哮喘动物模型建立过程中可通过降低氧化应激反应,促进IL-10的分泌从而调节免疫系统的平衡,改变Th1/Th2/Th17相关细胞因子的分泌格局发挥保护作用。

To explore the protective effect of bilirubin in asthmatic animal modeling, Balb/C mice were administrated with indi- rect bilirubin while sensitized and challenged by ovalbumin （OVA）. Airway inflammation degree was estimated using bron- choalveolar lavage fluid （BALF） cells counting and lung histopathology. The levels of antibody OVA slgE and the cytokines such as II.-4, IFN-y, II.-2, 11717, IL-10 and TGF-~ in the serum were determined using ELISA, and the expression of IL-17 in the lung tissue was tested by immunohistochemistry and the targets of oxidative stress were detected. The study found that the bilirubin level was elevated, inflammatory cells were reduced in lung tissue, the levels of OVA slgE and IL-4 were de- creased while IFN-7, IL-2, IL-10 and IFN-y / IL-4 increased, the expression of IL-17 in lung tissue and the oxidative stress ac- tion was decreased after the intervention of bilirubin in asthmatic mouse modeling. There was less influence on TGF-β. So bili- rubin played a protective role in asthmatic modeling by decreasing oxidative stress action and promoting the secretion of IL- 10 to regulate the balance of the immune system and the secretion pattern of Thl/Th2/Th17 eytokines.