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大豆核孔蛋白GmNup96基因的克隆及生物信息学分析 被引量：3

Cloning and Bioinformatics Analysis of Gm Nup96 in Soybean
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摘要 从大豆品种天隆1号的叶片中克隆Gm Nup96基因的c DNA序列,对其编码的氨基酸序列、蛋白质理化性质、一级结构、二级结构、亚细胞定位等进行了生物信息学分析。结果表明:Gm Nup96基因编码1 022个氨基酸,为具有一定亲水能力的酸性蛋白,不具有信号肽,相对分子量为116.199 7 k Da;二级结构预测结果显示,Gm Nup96序列存在α-螺旋(46.87%)、无规则卷曲(26.32%)、延伸链(17.03%)和β-转角(9.78%),并无其它二级结构;系统进化树分析表明,大豆Gm Nup96基因与野生大豆、芸豆、绿豆、红小豆之间的亲缘关系更近。 The cDNA of GmNup96 gene in Tianlong 1 was cloned, and its bioinformatics character was analyzed. The results showed that the gene encoded a 116. 199 7 kDa protein with 1 022 amino acids , which was a hydrophilic protein without signal peptide, and the GmNup96 protein contained a conserved autoproteolytic peptidase domain in the N terminus. Through the secondary structure prediction, GmNup96 protein contained alpha-helix （46. 87% ）, beta turn （ 9. 78% ）, extended strand （ 17.03% ） and random coil （26. 32% ）. Phylogenetic analysis of GmNup96 and its homologs with different plant species were conducted by the software MEGA 5.1. The results showed that GmNup96 from Glycine max were near with. Glycine soja, Phaseus vulgaris, Vigna radiata var. radinta and Vigna angularis in genetic relationship.
作者 纪丹丹 肖龙 孙培元 刘春燕 傅永福 陈庆山 JI Dan-dan XIAO Long SUN Pei-yuan LIU Chun-yan FU Yong-fu CHEN Qing-shan(College Agriculture, Northeast Agricultural University, Harbin 150030, China Qiqibar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161041 ,China Land Reclamation Research and Breeding Centre of Heilongjiang, Harbin 150050, China Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100000 ,China)
机构地区 东北农业大学农学院 黑龙江省农业科学院齐齐哈尔分院 黑龙江省农垦科研育种中心 中国农业科学院作物科学研究所
出处 《大豆科学》 CAS CSCD 北大核心 2016年第5期736-741,共6页 Soybean Science
基金 国家自然科学基金(31271747 31471516) 国家高技术发展计划"863计划"(2013AA102602) 东北农业大学博士启动基金
关键词 大豆 GmNup96 克隆 生物信息学分析 Soybean GmNup96 Gene cloning Bioinformatics analysis
分类号 S565.1 [农业科学—作物学]
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参考文献13

  • 1Rout M P, Aitchison J D, Suprapto A , et al. The yeast nuclear pore complex: Composition, architecture, and transport mecha- nism[J]. Journal of Cell Biology, 2000, 148(4) :635-651.
  • 2Cronshaw J M, Krutchinsky A N, Wenzhu Z, et al. Proteomic a- nalysis of the mammalian nuclear pore complex [ J ]. Journal ofCell Biology, 2002, 158(5) :915-927.
  • 3Fiserova J, Kiseleva E, Goldberg M W. Nuclear envelope and nu- clear pore complex structure and organization in tobacco BY-2 cells [J]. Plant Journal, 2009, 59(2) :243-255.
  • 4Goldberg M W, Allen T D. The nuclear pore complex and lamina: Three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy[ J]. Journal of Mo- lecular Biology, 1996, 257(4) :848-865.
  • 5Roberts K, Northcote D H. Structure of the nuclear pore in higher plants[J]. Nature, 1970, 228(5269) :385-386.
  • 6Yuelin Z, Xin L, A putative nucleoporin 96 is required for both basal defense and constitutive resistance responses mediated by suppressor of nprl-1, constitutive 1 [ J ]. Plant Cell, 2005, 17 (4) :1306-1316.
  • 7Geraint P, Sally W, Alex C, et al. The Arabidopsis supressor of auxin resistance proteins are nucleoporins with an important role in hormone signaling and development [ J ] . Plant Cell, 2006, 18 ( 7 ) : 1590-1603.
  • 8Yannick J, Chareerat M, Veley K M, et al. The nuclear pore pro- tein AtTPR is required for RNA homeostasis, flowering time, and auxin signaling [ J ] . Plant Physiology, 2007, 144 ( 3 ) : 1383-1390.
  • 9Xianfeng M X, Annkatrin R, Sivaramakrishnan M, et al. Nuclear pore anchor, the Arabidopsis homolog of Tpr/Mlpl/Mlp2/megator,is involved in mRNA export and SUMO homeostasis and affects di- verse aspects of plant development [ J ]. Plant Cell, 2007,19 (5) : 1537-1548.
  • 10Dong C H, I-tu X, Tang W, et al. A putative Arabidopsis nucleo- porin, AtNUP160, is critical for RNA export and required for plant tolerance to cold stress [ J ]. Molecular & Cellular Biology,2006, 26(24) :9533-9543.

二级参考文献16

  • 1Fornara F,de Montaigu A, Coupland G. SnapShot : Control of flowering in Arabidopsis [ J ]. Ceil,2010,141 :550,550e1-2.
  • 2Andres F, Coupland G. The genetic basis of flowering responses to seasonal cues [J]. Nat. Rev. Genet. ,2012,13:627-639.
  • 3Rout M P, Aitchison J D, Suprapto A, et al.. The yeast nuclear pore complex : Composition, architecture, and transport mechanism [J]. J. Cell Biol., 2000,148:635-651.
  • 4Cronshaw J M, Krutchinsky A N, Zhang W, et al.. Proteomic analysis of the mammalian nuclear pore complex [ J ]. J. Cell Biol., 2002,158 : 915-927.
  • 5Fisemva J, Kiseleva E, Goldberg M W. Nuclear envelope and nuclear pore complex structure and organization in tobacco BY- 2 cells [ J]. Plant J.,2009,59:243-255.
  • 6Goldberg M W, Allen T D. The nuclear pore complex and lamina: Three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy [ J]. J. Mol. Biol., 1996,257:848-865.
  • 7Roberts K, Northcote D H. Structure of the nuclear pore in higher plants [ J ]. Nature, 1970,228 : 385- 386.
  • 8Tran E J, Wente S R. Dynamic nuclear pore complexes : Life on the edge [ J ]. Cell, 2006,125 : 1041-1053.
  • 9Wiermer M, Cheng Y T, Imkampe J, et al.. Putative members of the Arabidopsis Nupl07-160 nuclear pore sub-complex contribute to pathogen defense [ J]. Plant J. , 2012,70:796 - 808.
  • 10Roth C, Wiermer M. Nucleoporins Nupl60 and Sehl are required for disease resistance in Arabidopsis [ J ]. Plant Signal. Behav. ,2012,7 : 1212-1214.

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大豆科学
2016年 第5期

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