摘要
Gs WRKY20基因是从野生大豆中挖掘到的非生物胁迫相关基因,该基因可以提高转基因拟南芥与苜蓿的抗旱性,并使拟南芥提前开花。栽培大豆中的Gm WRKY20是Gs WRKY20基因的同源基因,序列相似度达到99%。利用实时荧光定量PCR分析栽培大豆中Gm WRKY20基因表达特性,结果表明:Gm WRKY20基因在不同栽培品种间的表达没有显著差异;仅在大豆发育的特定阶段上调表达,其上调表达与大豆开花的起始相关;主要表达部位在叶片;不受光周期影响,但受到多种非生物胁迫影响。
GsWRKY20 was identified from Glycine soja as an abiotic stress related gene in our previous studies. Overexpression of GsWKY20 could enhance the drought tolerance of transgenic Arabidopsis or Alfalfa, and promote the flowering of transgenic Arabidopsis. GsWRKY20 encodes a WRKY-type transcription factor, with 99% identity to GmWRKY20 from Glycine max. In order to fully investigate the role and the possible molecular mechanism of GmWRKY20 in soybean, the expression of Gm- WRKY20 in cultivated soybean was fully studied by real-time RT-PCR. The results showed that the expression of GmWRKY20 did not show obvious differences among different soybean cultivars, and high expression of GmWRKY20 was observed before flowering, suggesting that the high expression of GmWRKY20 might play an important role in starting flowering. GmWRKY20 did not response to photoperiod but response to multiple abiotic stresses at transcript level.
作者
宁文峰
庞添
杨艳玲
张超
柏锡
NING Wen-feng PANG Tian YANG Yah-ling ZHANG Chao BAI Xi(College of Life Science, Northeast Agricultural University, Harbin 150030, China Qiqihar Horticultural Research Institute, Qiqihar 161006, China)
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第5期748-753,共6页
Soybean Science
基金
国家自然科学基金(31371544)
转基因生物新品种培育重大专项(2016ZX08004-002)
