pre-miR-27a基因rs895819多态性与大肠癌易感性的Meta分析

Meta-analysis of the association between pre-miR-27a rs895819 polymorphism and susceptibility to colorectal cancer

目的近年来，有关pre-miR-27a基因rs895819位点多态性与大肠癌易感性关系的研究日益增多，但结论并不一致。本研究从循证医学角度，综合评价pre-miR-27a基因rs895819位点多态性与大肠癌易感性的关系。方法通过检索数据库PubMed、Cochrane Library、EMBASE以及中国知网数据库、万方数据库，收集有关pre-miR-27a基因rs895819位点多态性与大肠癌罹患风险关系的病例一对照研究。检索时间1998-01-01-2015-11-01。依据文献纳入及排除标准筛选相关文献，提取基本数据信息并进行文献质量评估。采用Stata 12．0软件行Meta分析，计算合并0R值和95％CI，并进一步行亚组分析和敏感性分析。结果最终纳入6个病例-对照研究（包括2025例大肠癌患者和2320例非肿瘤对照者）。Meta分析结果显示，pre-miR-27a基因rs895819多态性与大肠癌罹患风险具有显著的相关性，G vs A：0R=1．18，95％CI=1．08～1．30；GG vs AA／AG：OR=1．52，95％CI=1．26～1．97；GG vs AA=OR=1．53，95％CI=1．26～1．86，P值均〈0．05。亚组分析结果发现，亚洲人群也有相似的结论，G vs A：OR=1．21，95％CI=1．09～1．35；AG／GG vs AA：OR=1．15，95％CI=1．00～1．32；GG vs AA／AG：0R=1．57，95％CI=1．27～1．93；GG vs AA：OR=1．59，95％CI=1．27～1．98，P值均〈0．05。结论pre-miR-27a基因rs895819多态性与大肠癌易感性之间具有相关性，并且GG基因型具有增加罹患大肠癌的风险。

OBJECTIVE In recent years, there were increasing numbers of studies focused on the relationship be- tween pre-miR-27a rs895819 polymorphism and the risk of colorectal cancer, but the conclusion was inconsistent. This study aimed to evaluate the association between pre-miR-27a rs895819 polymorphism and susceptibility to coloctectal cancer by Meta-analysis. METHODS A comprehensive electronic search for relvent papers was conducted in PubMed,Co- chrane Library, Embase, China Knowledge Resource Integrated Database,Wan fang Database. The case-control studies a- bout the association between pre-miR-27a rs895819 polymorphism and risk of coloctectal cancer were included according to the inclusion and exclusion criteria. We based our time on literature retrieval from inception to November 1 2015. After extracted the information data and evaluated the quality of literatures, the meta-analysis was conducted using Stata 12.0 software, with calculating odds ratio and 95 % confidence interval and followed subgroup analysis and sensitivity analysis. RESULTS Ultimately,a total of six studies involving 2 025 CRC cases and 2 320 healthy controls were found to be eligible for our meta-analysis. In Meta-analysis, significantly increased cancer risks were found in three genetic models in the overall analysis（G vs A,OR= 1.18,95%CI= 1.08-1.30;OR=1. 53,95%CI=1. 26-1. 86）. In the subgroup analysis by ethnicity, similar results were found among Asians in four GG vs AA/AG,OR= 1.52,95%CI= 1.26-1.97%GG vs AA, genetic models （G vs A,OR=1. 21,95%CI=1.09-1.35%AG/GG vs AA,OR=1. 15,95%CI=1.00-1.32%GG vs AA/ AG,OR=1. 57,95%CI=1.27-1.93,GG vs AA,OR=1. 59,95%CI=1.27-1.98）,P〈0.05. CONCLUSIONS There is a significant association between pre-miR-27a rs895819 polymorphism and susceptibility to coloctectal cancer. The GG genotype of rs895819 in pre-miR-27a gene might be associated with an increased risk of coloctectal cancer.