有蹄类家畜诱导多能性干细胞(iPSCs)研究进展

Progress of the Induced Pluripotent Stem Cells in Domesticated Ungulates

当今,学者已经在啮齿类和灵长类动物中建立了胚胎干细胞系,然而在有蹄类家畜中还没有获得成功。诱导重编程技术的建立为再生医学的快速发展以及农业动物繁育技术体系的革新提供了重要的技术支持,也为获得有蹄类家畜多能干细胞提供了新的思路和方法。目前,虽然在猪、牛、绵羊、山羊和马中都获得了诱导多能性干细胞(induced pluripotent stem cells,i PSCs),但是其在体外培养及细胞特性等方面与啮齿类和灵长类i PSCs还有较大差距。该文主要对有蹄类家畜i PSCs研究的现状、存在的问题及解决思路进行系统综述,为进一步推进有蹄类家畜i PSCs的研究提供理论支持。

Establishment of embryonic stem cell lines has been successful in mouse and human, but not in farm animals. Development of induced reprogramming technology has tremendous potential applications in regenerative medicine, animal breeding. It also offers an alternative approach for generation of pluripotent stem cells in domesticated ungulates. Recently, although porcine, bovine, ovine, caprine and equine iPSCs were derived from their somatic cells, compare to rodent and primate iPSCs, there were the gap in in vitro cultivation and cellular characteristics of ungulates iPSCs. As theoretical supports for iPSCs research in future, the aim of this review was to provide a systematic overview on iPSCs generation, substantial problems and solution in domesticated ungulates.