低氧对体外人软骨终板干细胞增殖、衰老、凋亡的影响

Effects of Hypoxia on the Growth, Senescence and Apoptosis of Human Cartilage Endplate Derived Stem Cells In Vitro

该文应用原代培养的方法获得人软骨终板干细胞(cartilage endplate derived stem cells,CESCs)。采用水溶性四唑盐(WST)-1法、Edu掺入法、β-半乳糖苷酶染色法、流式细胞术检测低氧(1%O2)刺激对CESCs细胞增殖、衰老、凋亡以及细胞周期的影响。结果显示,与常氧(21%O2)组相比较,低氧刺激对CESCs增殖活性有显著的促进作用,低氧处理72 h后,CESCs的增殖活性增加最为显著,低氧刺激能够极为显著的抑制CESCs的衰老,低氧对CESCs的凋亡同样具有极为显著的抑制作用;低氧影响CESCs的细胞周期,呈现出G1期细胞比例先增加后减少和(S+G2/M)期细胞比例先减少后增加的趋势。结果表明,低氧刺激能够促进CESCs增殖、抑制细胞衰老和凋亡。

In this study, we obtained human cartilage endplate derived stem cells （CESCs） via primary culture. The effects of hypoxia （1% 02） on growth, senescence, apoptosis and cell cycle of CESCs were investigated by WST-1 cell proliferation and cytotoxicity assay kit, Edu cell proliferation assay kit, 13-galactosidase staining, flow cytometry, respectively. The results showed that proliferation of CESCs was significantly promoted, senescence and apoptosis of CESCs were significantly inhibited cultured hypoxia for 72 h, compared with the normoxia （21% 02） group. Hypoxia culture affected the cell cycle of CESCs, cell proportion of G, phase was firstly increased then decreased, and the cell proportion of S and G2/M phases were firstly decreased then increased. The results demonstrated that hypoxia could promote CESCs proliferation and inhibit CESCs senescence and apoptosis.