基因芯片技术分析转SlNAC10基因拟南芥非生物胁迫相关差异表达基因

Microarray Analysis of Differentially Expressed Genes Related to Abiotic Stress in SlNAC10-Transgenic Arabidopsis

NAC(NAM、ATAF1/2和CUC2)转录因子是植物特有的转录调控因子,在植物的器官建成、生长发育以及抵御非生物胁迫等方面发挥着至关重要的作用。该文利用基因芯片技术筛选转Sl NAC10基因拟南芥和野生型拟南芥非生物胁迫抗性相关差异表达基因,并通过实时荧光定量PCR对部分差异表达基因进行验证。芯片结果显示,差异表达2倍以上的基因有4 054个,其中与非生物胁迫相关基因有15个,与非生物胁迫相关的转录因子基因有14个,这些基因参与应答渗透胁迫、响应高盐、冷、热、高光强等胁迫。对差异表达2倍以上的基因进行GO(Gene Ontology)分析和KEGG(Kyoto Encyclopedia of Genes and Genomes)分析,发现这些基因在非生物胁迫相关的13个注释中富集,涉及相关代谢途径96个,其中包括植物激素信号转导、精氨酸和脯氨酸代谢、吲哚生物碱合成、谷胱甘肽代谢等。以上结果表明,SlNAC10可直接或间接调控多种下游基因的表达,提高植物抵御非生物胁迫的能力。

NACs （NAM, ATAF1/2 and CUC2） were plant specific transcription factors and played essen- tial roles in plant morphogenesis, growth and development, and abiotic stress responses. In this study, microarray was used to analyze differentially expressed genes related abiotic stress in the wild-type （WT） and SlNAClO-transgenic Arabidopsis （L1）, and the expression patterns of selected differentially expressed genes were further identified by quantitative Real-time PCR. A total of 4 054 differentially expressed genes were found, 15 of them were related to abiotic stress, and 14 were abiotic stress-related TFs. These genes were involved in responding to osmotic stress, high salinity, cold, heat, high light intensity respectively. The differentially expressed genes were subjected to GO （Gene Ontology） analysis and KEGG （Kyoto Encyclopedia of Genes and Genomes） pathways enrichment analysis. These genes enriched in 13 annotations related to abiotic stress and involved in 96 metabolic pathways, including plant hormone signal transduction, arginine and proline metabolism, indole alkaloid biosynthesis and glutathione metabolism. These results indicated that S1NAC 10 regulated the expression of downstream genes directly or indi- rectly and enhanced plant resistance to abiotic stress .