米曲霉外源表达系统研究进展

Advances in Research of Aspergillus oryzae as a Host of Heterologous Protein Expression

丝状真菌米曲霉是发酵工业的重要菌种,具有强大的蛋白分泌能力和较高的食品安全性,可作为表达外源蛋白的细胞工厂。近年来,米曲霉全基因组序列的测序完成和基于表达序列标签的基因组学研究,为深入研究米曲霉外源表达系统提供了条件。从基因组学进展、遗传转化体系等方面综述了米曲霉作为外源蛋白表达宿主的研究进展。针对米曲霉在外源蛋白表达中存在的瓶颈,提出构建蛋白酶缺陷株、使用强启动子、融合表达等策略,以提高外源蛋白的表达和产量。最后介绍了米曲霉表达系统的应用,利用米曲霉代谢工程菌生产工业用酶和次级代谢产品具有良好的前景。

Aspergillus oryzae is a very important fungus in the fermentation of traditional foods. Filamentous fungus Aspergillus oryzae has been utilized as a cell factory for heterologous protein production because of its high protein secretory capacity and food-safety properties. Recently completed genomic studies using expressed sequence tag（ EST） analyses and whole-genome sequencing are quickly expanding the industrial potential of A.oryzae in biotechnology. The advances in genomics and transformation system which support the idea that A.oryzae is an ideal production hosts was focused. Whereas there are bottlenecks during heterologous proteins production in A.oryzae compared to high yields of homologous proteins. The strategies for improving heterologous protein production such as disruption of local gene,promoter and fusion expression were also discussed. Finally,application and high potential in enzymes and secondary metabolite production of the engineering A. oryzae were also reviewed.