海三棱藨草的组织培养与快繁体系

A Rapid Propagation System for Scirpus × mariqueter

以海三棱藨草(Scirpus×mariqueter)成熟种子为外植体,通过无菌萌发、丛生芽诱导、增殖、壮苗、生根和移栽等过程,建立了海三棱藨草的无菌快繁体系。结果表明:丛生芽诱导和增殖的最适培养基为MS+2.0 mg·L^(–1) 6-BA+0.002mg·L^(–1) TDZ+0.2 mg·L^(–1) IBA;壮苗最适培养基为1/2MS+0.05 mg·L^(–1) 6-BA+0.01 mg·L^(–1) IBA;生根最适培养基为1/2MS+0.2 mg·L^(–1) IBA;最适培养温度为30°C;再生苗移入珍珠岩:草炭:蛭石=1:1:1(体积比)的混合基质中,移栽成活率可达85%以上。生根培养阶段选用容积较大的塑料容器育苗,可以降低生产成本和提高生产效率。

The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS＋2.0 mg·L^–1 6-BA＋0.002 mg·L^–1 TDZ＋0.2 mg·L^–1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS＋0.05 mg·L^–1 6-BA＋0.01 mg·L^–1 IBA; the optimal medium for rooting was 1/2MS＋0.2 mg·L^–1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1：1：1, and the survival rate was 〉85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.