小尺度空间果生刺盘孢遗传多样性分析

A fine-scale genetic analyses of Colletotrichum fructicola strains from Camellia oleifera

本研究将来自16个油茶品种的247个Colletotrichum fructicola的多基因序列进行遗传多样性分析,结果表明,247个C.fructicola多基因序列有21个单倍型,其中Hap2占总数的54.7%,Hap4占总数的34%,为两个主要的单倍型,来自所有16个油茶品种。基本上每一个油茶品种都存在C.fructicola独特的单倍型。遗传分化系数(Fst)表明不同品种来源菌株种群间存在一定的遗传分化。对所有品种种群进行中性检测及核苷酸不配对分析,发现小尺度空间的果生次盘孢菌经历大规模的种群扩张,群体间存在有效基因流。系统发育分析表明,来自不同品种的菌株散乱地分布在系统发育树中。

Genetic diversity analysis of 247 Colletotrichum fructicola strains from 16 Camellia oleifera varieties were conducted and 21 haplotypes were identified based on the combined sequences of ITS-CAL-GAPDH. A- mong these, haplotypes 2 and 4 are the two major haplotypes which account for 54.7% and 34%, respectively, and can be identified from all of the 16 C. oleifera varieties. Each variety has its own unique haplotypes of C. fructicola. The coefficient of genetic differentiation （Fst） indicated that there is a certain genetic differentiation among C. fructicola strains derived from different varieties. Neutrality tests and mismatch distribution analysis suggested that there existed large population expansion for fine-scale C. fructicola. The multi-gene phylogenetic analysis showed that strians from different varieties were scattered distribution in the phylogenetic tree.