miR-152在非小细胞肺癌患者血清中的表达及临床意义

Serum microRNA-152 expression and its clinical significance in patients with non-small cell lung cancer

目的 通过检测非小细胞肺癌（NSCLC）患者血清中microRNA-152（miR-152）的水平,探讨其与NSCLC的关系。方法 采用Real time-PCR法检测61例NSCLC患者和80例健康对照者血清miR-152的表达水平,并分析其与临床病理指标间的关系。结果 NSCLC组血清miR-152的表达水平显著降低（P〈0.01）。且其表达量与患者吸烟史和TNM分期有关,有吸烟史的NSCLC晚期的患者表达更低（P〈0.05）,而在不同年龄、性别、组织学类型、肿瘤分化间差异无统计学意义（P〉0.05）。根据miR-152的表达水平绘制ROC曲线,曲线下面积为0.820（95%CI=0.752~0.889）,临界值取0.48时,灵敏度和特异度分别为63.80%和90.20%。结论 NSCLC患者血清中miR-152表达低,且与肿瘤分化程度相关,miR-152可能作为NSCLC诊断的特异肿瘤标志物。

Objective Tto investigate the expression of microRNA-152（miR-152）in the serum of patients with non-small cell lung cancer（NSCLC） ,and to explore the clinical significance of NSCLC. Methods The relative expression levels of miR-152 in serum of 61 NSCLC patients and 80 healthy individuals were assessed by quantitative real-time PCR（RT-PCR）,and the clinical pathological parameters was analyzed. Results The expression of miR-152 was significantly lower in the serum of NSCLC than that of controls（P〈0.01）. Down regulation of miR-152 was found to be correted with the clinicopathological features such as smoking histroy and TNM staging（P〈0.05）, but there was no significant difference in different age, sex, histological type and tumor differentiation（P〉0.05）. The ROC curve for miR-152 revealed a strong diagnostic performance, the value of the area under ROC（AUC- ROC）was 0. 820（95%CI：0. 752-0. 889） ,optimal sensitivity and specificity were 63.80% and 90.20% when 0.48 was selected as the critical value. Conclusion It was found that rniR-152 is significantly reduced in serum samples of NSCLC patients,and the data suggests that it may be a particular biomarker for the clinical diagnosis of NSCLC.