强力五虎合剂水提工艺指标性成分测定方法的建立

Determining Index Component of Wuhu Mixture by Water Extraction

目的：建立强力五虎合剂中指标性成分苦杏仁苷、盐酸麻黄碱的含量测定方法。方法：应用HPLC检测法（LC-20A岛津高效液相色谱仪），色谱柱为WondaSilC18（4．6mm×250mm，5μm），流动相分别以甲醇-乙腈-0．1％磷酸（16：4：80），在207nm波长测定苦杏仁苷；O．1％磷酸一乙腈（95：5），在20711113．波长测定盐酸麻黄碱。结果：苦杏仁苷在0．100-0．800μg（r=0．9994），盐酸麻黄碱在0．008-0．048I．Lg（r=0．9999）之间的范围内呈现良好的线性关系。样品中苦杏仁苷平均回收率为99．5l％，RSD=2．51％；盐酸麻黄碱平均回收率为97．47％，RSD=1．75％。结论：该方法结果准确，灵敏度高，重现性好，可用于控制强力五虎合剂的质量。

Objective ：To establish a method for determining Wuhu mixture index components including amygdalin and ephed- rine hydrochloride. Methods：HPLC was used（ LC -20A Daojin high performance liquid chromatograph） ：column chromatography WondaSil C is （4.6 mm× 250 mm,5 μm）;methanol acetonitrile- 0.1% phosphoric acid 16：4：80, at 207 nm wavelength to de- termine amygdalin and 0.1% phosphoric acid and acetonitrile （95：5）, at 207 nm wavelength to determine ephedrine hydrochlo- ride. Results ： Amygdalin in 0. 100 - 0.800 μg （ r = 0.9994）, ephedrine hydrochloride in 0.008 - 0.048 μg （ r = 0. 9999 ） presen- ted good linear relationship. The average recovery rate of amygdalin samples were 99.51%, RSD = 2.51%. And that of ephedrine hydrochloride samples was 97.47%, RSD - 1.75 %. Conclusion：The method is accurate, with high sensitivity and good reproduc- ibility and can be used for quality control of Wuhu mixture.