摘要
目的:建立HPLC方法测定不同提取时间对钩藤中7种生物碱含量的变化的影响。方法:采用HPLC方法对不同提取时间钩藤供试品中的钩藤碱和异钩藤碱含量变化进行测定,并记录其中七种生物碱峰面积的变化规律。结果:钩藤药材提取50 min时7种生物碱成分提取量最高,且随加热时间的延长各成分含量均逐渐减少或不变。结论:钩藤总生物碱性质不稳定,提取时间过长容易破坏,50 min为最佳提取时间。
Objective ： To establish HPLC method for 7 kinds of alkaloid contents of different decocting time of Rhynchophylla. Methods ~ By using the HPLC method to determine 7 kinds of alkaloid contents changes in different decocting time. Results ： Extrac- ting for 50 rain,7 kinds of alkaloids extraction amounts were the highest. With the decocting time increasing, the content of each component was decreased or unchanged. Conclusion：Rhynchophylla total alkaloids are unstable and if the decocting time is too long, it is easy to be damaged. Fifty min is the best decocting time.
出处
《辽宁中医杂志》
CAS
北大核心
2016年第9期1946-1947,I0002,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家"重大新药创制"科技重大专项(2012ZX09102201-004)
