脾气虚证大鼠与鱼藤酮损伤大鼠之间下丘脑cAMP/PKA信号通路变化的研究

cAMP / PKA Signal Transduction Pathway Change of Hypothalamus between Spleen Qi Deficiency Rats and Rotenone Damage Rats

目的:探讨脾气虚证大鼠与鱼藤酮损伤大鼠之间下丘脑c AMP/PKA信号通路变化。方法:30只SPF级雄性大鼠,随机分为正常组,脾气虚组,鱼藤酮高剂量组(2.0 m L/kg),中剂量组(1.5 m L/kg),低剂量组(1.0 m L/kg),每组6只。采用ELISA法检测各组大鼠下丘脑组织c AMP活性,Real Time PCR法和Westernblot法检测蛋白激酶(cyclic-AMP-dependent protein kinase,PKA)、糖原磷酸化酶(glyeogenphospholase,Gp)、磷酸化酶激酶(phosphorylase kinase,PHK)的mRNA及其表达量。结果:与正常组大鼠比较,脾气虚证组及鱼藤酮3组大鼠的下丘脑组织c AMP活性及PKA、Gp、PHK mRNA和蛋白的表达均有不同程度的减弱,鱼藤酮中剂量组的结果与脾气虚证组最为相似。结论:脾气虚证大鼠与鱼藤酮损伤大鼠之间下丘脑c AMP/PKA信号通路的变化存在相关性,其中c AMP-PKA信号通路可能参与调控。

Objective：To discuss the cAMP/PKA signal transduction pathway change of the hypothalamus between spleen Qi deficiency rats and rotenone damage rats. Methods ： Thirty male SD SPF rats were randomly assigned into 5 groups,including con- trol group, spleen Qi deficiency group, rotenone high - dose group （2.0 mL/kg）, middle - dose group （ 1.5 mL/kg） and low - dose group（ 1.0 mL/kg）,6 rats in each group. And then test the cAMP activity and PKA,PHK,Gp mRNA and protein expression of the hypotbalamus tissue. Results：Results ：The hypothalamus tissue cAMP activity and PKA, PHK, Gp mRNA and protein expres- sion of spleen Qi deficiency rats and 3 rotenone groups were weakened compared with the control group. The results of rotenone middle- dose group was similar to the spleen Qi deficiency group. Conclusion： The cAMP/PKA signal transduction pathway change of the hypothalamus between spleen Qi deficiency rats and rotenone damage rats has a remarkable correlation. In addition, the cAMP/PKA signal transduction pathway is possibly involved in rezulation.