摘要
应用光学技术对荧光标记的组织切片样品进行成像观察是生物学研究的常规手段。本文主要介绍如何制备用于荧光显微镜和激光共聚焦显微镜成像的脑组织石蜡切片免疫荧光双重标记技术。
Observation of fluorescent labeled tissue sections by optical technique is a common method for biological research. In this paper,we mainly introduce the double labeling technique of paraffin sections of brain tissue by fluorescence microscope and laser scanning confocal microscopy.
作者
宋兴辉
徐阳
陈静瑶
金晓琴
朱有法
SONG Xing- hui XU Yang CHEN Jing- yao JIN Xiao- qin ZHU You- fa(Core Facilities,Zhejiang University School of Medicine,Hangzhou Zhejiang 310058 The First Affiliated Hospital of Medical School of Zhejiang University, Zhejiang Hangzhou 310058 ,China)
出处
《电子显微学报》
CAS
CSCD
2016年第5期459-461,共3页
Journal of Chinese Electron Microscopy Society
基金
浙江省科技厅公益项目(分析测试类)(No.2014C37067)
浙江大学实验技术研究项目(No.SYB201410)
中央高校基本科研业务费专项资金(No.2016QNA7021)
关键词
脑组织
石蜡切片
免疫荧光双重标记
显微镜成像
brain tissue
paraffin section
double labeled immunofluorescence
microscope imaging
