多倍体金线莲一次性成苗及移栽

One-step seedling formation and transplanting of polyploid Anoectochilus roxburghii

筛选多倍体金线莲一次性成苗的最佳培养基配方并探讨不同基质对移栽苗生长的影响.结果表明：MS＋1.0mg·L^-16-BA＋1.5mg·L^-1NAA＋150g·L^-1土豆＋3%蔗糖＋0.65%琼脂＋0.15%活性炭为最佳-次性成苗培养基配方;经120d培养即可获得生长、生根同步完成的组培瓶苗,具有长势一致（株高5-7cm），茎节多且粗（茎粗1.19cm），根系发达（每株2-3条根）等优势;最佳移栽基质为：泥炭土：碎松树皮=1：1（体积比）,移栽苗成活率（92%）最高,长势最好,植株增高（1.53cm）、增重（0.43g）均达到最大,折干率（13.31%）相对较高.

Polyploid Anoectochilus roxburghii has bigger vegetative organs than its diploid plant, such as roots, stems, leaves, besides which it also has higher growth rate and organics synthesis rate, stronger resistance, and higher medicinal ingredients. In this study, one-step seedling for^nation culture medium and transplanting of polyploid A.roxburghii were studied. The results showed： MS ＋ 1.0 mg · L ^- 1 6-BA＋1.5 mg · L^- 1 NAA＋150 g · L^- 1 potato. mud＋3% sucrose＋0.65% agar＋0.15% activated carbon is the optimum culture medium for one-step seedling formation. Fully growing plants formed from one stem’ 5-7 cm in height’ 1.19 cm in stem diameter’ and 2-3 healthy roots can be gained after 120 drs cultivation. The optimum transplanting medium is matrix peat · bark= 1·1 （v · v） with 92% transplanting survival rate’ 1.53 cm increase in height’ 0.43 g increase in weight’ and 13.31% dry discount rate. To solve transplanting problem of polyploid Anoectochilus roxburghii because of its larger vegetative organs and high growth rate ’ one-step seedling formation culture medium and transplanting of polyploid A. roxburghii were studied. The results showed that MS with 1.0 mg · L^-1 6 -BA· 1.5 mg · L^-1 NAA·150 g · L^-1 potato mud’ 3% sucrose’ 0.65% agar and 0.15% activated carbon was the optimal culture medium for one-step seedling formation. After 120 d cultivation’ plants fully developed to 5-7 cm in height’ 1.19 cm in stem diameter’ and 2？3 healthy roots. The optimum transplanting medium was matrix peat ： bark = 1 ： 1 （v ： v） with 92% transplanting sun,ival rate. Moreover’ it resulted in 1.53 cm increase in height’ 0.43 g increase in weight’ and high dry discount rate at 13.31%.